how can I calculate the concentration and mass of the active substance of the drug by lambert beer law with give data?
average mass of the drug- 0,2961g
masses of pills: 0,2961g; 0,2856g; 0,2874g- active substance- 250mg
masses of pills used: 0,0118g; 0,0114g; 0,0115g- active substance 9,96mg; 9,98mg; 10mg
absorbance: 0,4344; 0,4423; 0,4126
and the extinction coefficients E1%1cm= 537
this is how the experiment goes: the amount of powdered tablets corresponding to approximately 0.01 g of active substance put into the flask 10 ml, add 8 ml ethanol (95%), heat the content to 40-50 oC stir, cool, make up the volume with ethanol to the mark. Filter the contents of the flask - solution primary (B). Then, to the volumetric flask 10 ml thoroughly put 0.1 ml of solution (B), make up the volume to the mark with ethanol and mix thoroughly -(c)
You'll need to add a line of best fit to the data points and determine the equation for the line. The equation should be in y=mx + b form. So if you substract your y-intercept from the absorbance and divide by the slope, you are finding the concentration of your drug sample
Firstly you have to determine concentration of drug released by using formula
Y =mX +/- C
Y is absorbance, m is the slope, C is intercept, X is concentration (mcg/ml)
After the determination of concentration, calculate amount of drug released by using formula
Amount of drug released (mg)= [Concentration X Dilution factor X Volume of Dissolution medium] / 1000
and at last, by using formula determine the drug release
% Drug release = Amt of drug released (mg) X 100 / Dose (mg)
Dear Julia Jurgielewicz,
I published a paper some time ago, which may probably have similarity to your concern. Please refer to Section 3.1 of this paper:
Scattering Effects in Laser Attenuation System for Measurement of Droplet Number Density , Energy Procedia, 50 ( 2014 ) 79 – 86. Available at: Article Scattering Effects in Laser Attenuation System for Measureme...
I hope this would somewhat be helpful.
Shaharin
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