Which is the best material for an electromagnet core?

I need to build a continuous electromagnet, B=1-2 T, narrow hysteresis loop, good linearity (to have the flux density easily adjustable). Application - MeV electron beam deflection. I'll be...

27 April 2020 4,345 2 View

Why is RPP30 used as the reference gene for haploid genome copy number?

I heard from a colleague that RPP30 is a gene that is very rarely duplicated or deleted in cancer cells, but I haven't been able to find any reference for that. Does anyone know of any research...

16 December 2018 8,251 1 View

Has anyone got diagonal false positives in ddPCR?

Since some time I've been getting quite a few droplets in my NTCs that have high and similar ch1 and ch2 amplitude, i.e. they find themselves on the diagonal of 2D plots. It didn't used to happen...

24 January 2017 3,503 7 View

Can anyone suggest method of sample preparation for GC-MS determination of PAHs in soil?

13 July 2016 7,224 5 View

Is there possibility to obtain electronic copy of the 1897 G. Longchamps paper ?

01 June 2016 4,522 3 View

Why in soft agar transformation assay, are NIH 3T3 cells (which are not transfected) forming colonies?

I start to run soft agar colony formation assay. I used NIH 3T3 cells not transfected, transfected with few variants of LTK, empty vector as control and KRAS expression construct as positive...

06 April 2015 4,986 6 View

What do we know about Initial Singularity?

Assuming that the Big Bang theory reflects the actual chronology of "The Birth of the Universe", imposes the question of whether The Big Bang makes sense without existence of The Initial...

12 October 2013 9,192 85 View

No title

I am trying to estimate the most likely number ok K using MCMC (Markov Chain Monte-Carlo Inference Of Clusters From Genotype Data) function in Geneland R-package by Gilles Guillot et al. I am a...

01 January 1970 6,537 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

How much cell pressure and back pressure should I apply to start for triaxial test for different types of soil?

We have recently purchased a triaxial test instrument (MATEST) for testing different types of soil. After installing software, we are facing a issue of saturation where the samples does not...

23 February 2021 7,194 4 View

What will be the possible analysis plan for descriptive outcome?

Hi everyone I am busy with a Systematic review. Unfortunately, my protocol has been returned back because of my analysis plan that will not answer my question. Can you please advise on the...

02 February 2021 6,190 2 View

Are there any solutions for remote eye-tracking?

Since with the pandemic it doesn't look like we are going back to the lab soon, in my lab we were looking for solutions to conduct remote webcam-based eye-tracking. I've been looking at...

31 January 2021 7,315 5 View

How to extract protein after trizol RNA and back extraction of genomic DNA?

I have limited amount of samples, so I am trying to get RNA, DNA and protein from the same samples. I used Trizol reagent for RNA isolation, then use back extraction buffer for genomic DNA...

13 January 2021 1,566 2 View

Are most-recent papers of randomized clinical trials needed to conduct a legitimate meta-analysis?

Are most-recent papers of randomized clinical trials needed to conduct a legitimate meta-analysis or decades back will still provide optimum results?

28 December 2020 9,258 3 View

I want to use multilayer feedforward neural network with error backpropagation algorithm. I have just 30 years monthly rainfall data. Is this enough??

I am new to neural networks and want to apply multilayer feedforward neural network with error back propagation algorithm for the rainfall prediction. I have just monthly rainfall data of 30...

28 December 2020 9,755 4 View

Can language alter our perception of crime?

I am looking for any resources which may be useful in a study I am conducting on the impacts that language may have on our perception of crimes. I will be using headlines which convey a particular...

23 December 2020 8,713 6 View

Is ag paste suitable for back contact on silicon wafer? or i should deposit al on the back surface using pvd or plasma sputtering?

Fabricating silicon solar cell

22 December 2020 4,906 7 View

Fluorescence spectra problem during protein ligand interaction??

I checked protein ligand interaction through Fluroscence, I keep my protein concentration constant with varying concentration of ligand at the small concentration 5uM the FLuroscence is peak is...

21 December 2020 8,609 4 View