Hi! I'm little new on this signaling pathway, but I'd like to test if certain compounds can activate Notch signaling on HUVECs. As a positive control I'm thinking on using Jagged1. Which downstream genes could be indicative of Notch activation on this cells? Any protein on the signaling cascade that could be measured too? Many thanks in advance!
Hi Enrique,
You can determine Notch pathway activation by measuring the mRNA levels of Hey1 and Hey2 target genes(RTqPCR).
Please see following link, the study by Estrach and collaborators on Notch pathway in HUVEC.
Article Laminin-Binding Integrins Induce Dll4 Expression and Notch S...
Best,
Elmina
RBP‐Jk luciferase reporter gene assays are available in SA Biosciences. Other than this assay, you can also measure a panel of notch target genes and look for target gene induction with notch activation, etc.
Chapter The Molecular Mechanism of Notch Activation
Dear Dr.Shanmugam,
The above chapter is really informative and could answer your question; if you can not access it, please send me your contact email and I will send it to you
regards,
Essam
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