Enrique Andres Sastre

4 Questions 1 Answers 0 Followers

Questions related from Enrique Andres Sastre

Which contrast agent would you choose to assess animal vasculature by histology in the context below?

Dear all, I would like to assess by histology the degree of vascularisation that occurs on a mouse bone after the local administration of a certain compound. I've thought to administer a contrast...

09 September 2019 7,359 1 View

How can I assess activation of Notch signaling in HUVECs?

Hi! I'm little new on this signaling pathway, but I'd like to test if certain compounds can activate Notch signaling on HUVECs. As a positive control I'm thinking on using Jagged1. Which...

01 January 2019 7,445 4 View

What to do with PVDF membrane after methanol activation (Western Blot)?

Hi everyone, I'm interested to perform a semidry transfer for a Western Blot. Right after activating the PVDF membrane for 30 seconds on pure methanol, do you directly place it on the gel, or do...

11 November 2017 3,454 6 View

Western Blot - Why does the voltage goes too high during a semi-dry trasfer with constant amperage?

Hi, I'm getting troubles with the transfer of my western blot. I'm performing a semi-dry transfer (BioRad transblot SD) with constant amperage (0.25 A) but then the voltage is increasing to 45V,...

08 August 2017 8,133 4 View