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Dear all, I would like to assess by histology the degree of vascularisation that occurs on a mouse bone after the local administration of a certain compound. I've thought to administer a contrast...
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Hi! I'm little new on this signaling pathway, but I'd like to test if certain compounds can activate Notch signaling on HUVECs. As a positive control I'm thinking on using Jagged1. Which...
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Hi everyone, I'm interested to perform a semidry transfer for a Western Blot. Right after activating the PVDF membrane for 30 seconds on pure methanol, do you directly place it on the gel, or do...
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Hi, I'm getting troubles with the transfer of my western blot. I'm performing a semi-dry transfer (BioRad transblot SD) with constant amperage (0.25 A) but then the voltage is increasing to 45V,...
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