I want to analyze vascular smooth muscle in tunica media or endothelial cells in tunica intima hypertrophy or hyperplasia that can change the ratio of tunica media/lumen diameter. Can anyone help?
My personal opinion is that haematoxylin-eosin staining is a good method for assessing histological specimen characteristics. However, with regards to vascular tree cells, weigert and movat staining could be more useful. In addition, the functional characteristics of the artery coats may be well determined by histochemical methods.
I suggest Mason`s trichromic staining in order to contrast the specimen. With that, you can perform an analysis of diameter with Image J or Fiji. Its easier with Mason because it allows more contrast among the different tissues.