Do you want to quantify it, identify the different pollens present or something else?

Yes i want to identify and quantify differnet pollens present

I don't know of a particular method for quantifying pollen, although perhaps a protein assay of some sort would work. Although, I'm sure you'd have to be careful about how you take samples for analysis.

Pollens have variable stuctures, so they can be identified by scanning electron microscopy. If you have a reasonable idea of what you're likely to find you can then match them with pictures on the web.

http://ngm.nationalgeographic.com/2009/12/pollen/img/04-composite-714.jpg

by Melissopalynology methods.

Normal microscopy. There are standards (at least we had a national one) depicting different pollen shapes. It needs practice!

You can follow the methods used by colleagues of UNAM, Mexco

http://dx.doi.org/10.2317/0022-8567(2007)80[377:MCOSMG]2.0.CO;2

This method is recommended by the International Commission for Bee Botany:

Ten grams of honey is dissolved in 20 ml of warm water (40 C).

The solution is centrifuged for 10 min at 2500 r/min, the supernatant solution is decanted, and the sediments are collected into a conical tube and treated with an acetolysis mixture (acetic anhydride : conc. sulphuric acid = 9:1 V/V) for approximately 30 min at room temperature.

After treatment with the acetolysis mixture, the sediments are rinsed with distilled water, centrifuge for 5 min at 2500 r/min, and preserve for study.