How can I analyse microcystin concentration in lake sediments using Elisa? Can anybody suggest the best extraction and analysis protocol?

13 February 2013 1 5K Report

I am curious to know how temporally the toxin concentration varies in lake sediments as it settles after release from cyanobacterial decay. Also, does it have any influence on setting up a toxic bloom?

Ammar Saleem

Just noticed your interesting question that you posted last year:

Our attempts to ELISA sediments failed due to matrix effect. This motivated us to develop an MS/MS method using MRM as a major approach. The paper is submitted in ACA and I will post the updates on this pioneering study as I get acceptance.

I am curious to know how did you solve your problem. Did ELISA help you at all?

Cheers!

Ammar

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