You can add His-tag to the end of your protein to produce such as 6x His-tagged recombinant protein (6 histidine add to the end of your protein). Then, there are commercial-available 6x His-tag antibody [see below example link]. You can use this antibody for Western, immunoassay......experiments. The antibody are considered as highly sensitive and specific for proteins bearing the histidine tag that they were raised against. 

Example: http://www.clontech.com/US/Products/Protein_Expression_and_Purification/Detection_of_Tagged_Proteins/Antibodies?gclid=Cj0KEQjwi-moBRDL4Omf9d_LndMBEiQAQtFf8wkDv4xqYR3JAvNf_LQYL6vENz5KmnK_mduS9N27OWAaApET8P8HAQ

Thank you Yuan-Yeu Yau, i already purified it by size exclusion. but i dont have antibody for it.

Anwar,

Have you tried raising the antibody against smaller portions of your chitinase, or just full-length?  We've successfully done that for some of our larger, more problematic proteins.  Do you intend to use this for western blotting or IPs?

Cheers,

Nick

Thank you Nicholas for your reply. I require it for western blot. further i did transformation of that gene in potato. i performed northern blot but i think doing western will be good.

I tried to rise the antibodies but both of time i was unable due to some of managerial problems.