It should be possible to induce germination also on slides or agar plates. I have already tried different methods: aqueous solution, film of water on slide, nutrient solution with dextrose, water-agar plates and so on. But no germination occurs (histochemical studies worked). Somewhat difficult is to realize 100 % relative humidity. Maybe that’s the problem. The objective is to test inhibiting effects of various plant extract samples compared to water.
Many thanks in advance!
Dear Marit Gillmeister
I attached three of my published papers on powdery mildew, I hope to be useful for your projects. Please do not hesitate to contact me about any question related to this subject.
Good luck
Jawad
Dear Marit Gillmeiste ,
Maybe you can try Formvar ® /based in vitro system.
You can check the method in papers:
Zhu M, Riederer M, Hildebrandt U. Very-long-chain aldehydes induce appressorium formation in ascospores of the wheat powdery mildew fungus Blumeria graminis[J]. Fungal Biology, 2017.
Hansjakob A, Bischof S, Bringmann G, et al. Very‐long‐chain aldehydes promote in vitro prepenetration processes of Blumeria graminis in a dose‐and chain length‐dependent manner[J]. New Phytologist, 2010, 188(4): 1039-1054.
Mo
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