How can Get good yield from DNA of Mycobacterium tuberculosis in sputum with good quality..?

More Nadir Musa Abuzeid's questions See All

What is the economic return to cities from hosting and organizing sporting events?

the economic return from hosting and organizing sporting events

09 June 2024 5,991 4 View

What are the modern management monitoring tools that contribute to improving job performance in sports institutions?

modern management monitoring tools performance in sports institutions

31 May 2024 2,466 0 View

What are the mechanisms for implementing strategic management in sports institutions?

The reality of applying strategic management in sports institutions

27 May 2024 2,341 3 View

What are the requirements for organizing sports events and their impact on sustainable development?

Requirements for organizing sports events and their impact on sustainable development

19 May 2024 2,142 1 View

Where is the sports diplomacy in sports bodies?

Sports diplomacy in sports bodies between reality and what is hoped for

20 April 2024 4,615 0 View

What are the mechanisms for using artificial intelligence to advance sports?

Mechanisms for using artificial intelligence to advance sports

14 April 2024 8,546 1 View

How important is mental training for youth players?

mental training youth players

03 April 2024 320 1 View

When the crystallite size estimated by XRD equal to grain size for thin films?

I think that the XRD for thin film also measured the grain size

28 March 2024 3,893 2 View

Sports and the freedom to express religious beliefs and their connection to the political side, where are the problems?

players restricted in expressing their religious opinions and beliefs linking them to the political aspect? Where is freedom of expression?

28 October 2023 4,637 1 View

Im looking for grid standards of low-power scale PV systems like domestic and rooftop systems, is there any work to suggest?

Dear all Im doing research on the field of PV systems connected to Low Voltage grid, especially low-power (under 500 W) installations such as rooftop systems. I would ask if the Fault ride...

14 March 2023 7,646 0 View

Do some Staphylococcus aureus strains have in vitro antimycobacterial activity ?

Some Staphylococcus aureus strains Inhibit the growth of Mycobacteria in Mueller Hinton Agar medium containing 10% OADC. Do some Staphylococcus aureus strains have in vitro antimycobacterial activity?

29 July 2024 10,023 2 View

Can I do IPTG induction on OD600=2.6 in TB?

I have been looking at papers for protein expression and trying to follow the process. I'm using TB medium to do the process, and the paper says to grow to an OD600 = 1.8 at 37 degrees, then let...

30 June 2024 6,502 5 View

How do i use researchgate to cunduct literature review ?

I am a first-time researcher in the field of nursing and i would like to conduct research about knowledge and attitude towards prevention and spread of tuberculosis.

11 June 2024 3,821 2 View

The gap in diagnosing intra-thoracic tuberculosis in children is not closed despite the advanncement in microbiology fields?

In 2024, childhood tuberculosis will still be a significant global public health issue, especially in underdeveloped nations (LMICS). It is true that the underestimation of the disease's extent...

31 May 2024 5,221 0 View

I'm not able to see the overexpression of my antitoxin on SDS PAGE of my chosen toxin-antitoxin system of mycobacterial species. Is there anything ?

I have been trying to check the overexpression of the antitoxin of my chosen TA system of mycobacterium sp. I have tried to induce the clone at 20, 25 and 37° C at 0.1, 1 and 10 mM concentration...

22 May 2024 1,334 0 View

Does anyone face this problem with a plasmid prepared from Midi-prep culture?

Hello, I have propagated a plasmid form a single colony (size around 15 kb) using small scale culture (2 ml of TB with selected Antibiotic) and purified it using Mini-prep kits ( FastGene Plasmid...

14 May 2024 6,123 2 View

How can I improve Gibson Assembly's efficiency?

In my undergraduate research project, I require an integrative plasmid containing GFP with an antibiotic resistance that can't be kanamycin. In our lab, all of our integrative-GFP-containing...

14 May 2024 9,861 3 View

Is there any method to do TDM for TB drug Ethambutol in human plasma by UHPLC with PDA detector?

We are standardizing chromatographic conditions for first-line TB drugs in our newly procured UHPLC system. For Ethambutol, we are not able to get dedicated chromatogram. Can anyone suggest any...

24 April 2024 9,859 0 View

Recently we are develping methods for Therapeutic drug monitoring of TB drugs, Need to know the required calculation for final estimation of drugs?

Hello, everyone. This is Dr Prerna, SR Biochemistry, NITRD, New Delhi. We are developing methods for Therapeutic drug monitoring of first line TB drugs in our laboratory. After utilizing suitable...

17 April 2024 9,598 0 View

FAD containing Protein purification?

I am trying to express a FAD containing enzyme of mycobacteria in E.coli. I am able to purify the protein which is slightly yellow in color but it seems that my FAD is all unbound to the protein....

16 April 2024 6,912 1 View

Sheilla Chebore

Have clear process by adhering to standard operating procedure depending on the extraction reagents and protocol

Saba A. Hussien

I add a file.

Jennifer Bowling

Quality of sputum is also very important. Sputum samples can be difficult to collect, and often will be mostly spit and not sputum. And of course a good DNA extraction protocol. Qiagen kits are expensive, but tend to be easy to use and work well.