I need a protocol for extracting ochratoxin A from green coffee beans for analysis on hplc, as well as the protocol for carrying out the calibr ?

ochratoxine A ; HPLC ; green coffee

29 December 2020 4,028 3 View

Could anybody give me information about room temperature degradation of silicone oil to silicon oxide?

Does anyone have any experience of room temperature degradation of silicone oil to silica in presence of acids?

26 December 2020 1,275 4 View

Should i add ATG in the beginning of my polypetide sequence when i want to insert it in a phagemid?

Hi I want to make a recombinant m13 phage displaying my peptide as a fusion peptide on its surface pVIII. Should i add ATG at the beginning when i want to insert it in phagmid vector? Previously,...

18 December 2020 9,931 2 View

What is the most recommended multispectral-UAV to be used for delineating the shoreline?

Hi there, I am looking to purchase an UAV with an attached multi-spectral camera to use its images in delineate the shoreline. I need to take a vertical geo-referenced image (if possible) and...

21 November 2020 1,283 3 View

How can i delaying a signal in Matlab script file without using simulink block (i want the instruction of delaying a signal in script file ) ?

How can i delaying a signal in Matlab script file without using simulink block? (i want the instruction of delaying a signal in script file )

27 October 2020 4,299 2 View

What would you do if MgSO4 did not fully dry your organic extract ?

Hi everyone, I used MgSO4 as a drying agent to suck all water droplets from my organic extract. However, even after adding MgSO4 three times and leaving it overnight, I still see some water...

14 September 2020 9,203 3 View

What multiphase model should be used to simulate the evaporation of an oil droplet in ANSYS FLUENT?

Hello, we have a sheet of Aluminium and some droplets of oil on it. The temperature of the sheet increases and the oil droplet evaporates in the air. The droplet has water and kerosene too and...

29 August 2020 2,268 3 View

How can I download Haiti earthquake datasets?

How can I download Haiti 2010 GeoEye Satellite images or any other datasets regarding this mentioned study area? Is there any FTP server or website to access? Or If anyone has this data set, I'm...

07 August 2020 4,872 3 View

How to activate the solid species tab in species transport in ANSYS Fluent?

Hello, I have to add a solid catalyst to the mixture but the tab is not activated. I have defined the solid material in the materials section but the list is showing the fluid materials only. How...

19 July 2020 4,930 3 View

How to make intervals in an ANSYS Fluent user defined function UDF to describe three different equations through time?

Hello, I have a plot shows the change of a quantity with time. How can we write a code in Fluent UDF to compile it ? I need a way to define the time intervals. From start to a specific moment, the...

30 May 2020 5,774 10 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View

How can I publish a manuscript in free or low cost journal with good H-Index?

Dear all I am going to publish my manuscript which is from my thesis in a good H-Index journal but I found them very expensive(I don't have financial support) based on the exchange currency of my...

28 February 2021 7,800 6 View

For modelling epoxy-based matrices (in composites), which model in ABAQUS is the most suitable, Concrete damaged plasticity or Drucker-Prager model?

I am working on developing a micromechanical FE model for predicting kink-band formation in UD composites. To model matrix plasticity, which model, out of Concrete Damaged Plasticity and...

28 February 2021 5,415 1 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Your thoughts on the spherical densiometer to estimate relative species canopy cover?

Hi biologists and biogeomorphologists, I want to characterize vegetation plots on a transect that follows a successionnal gradient from a river bed to a mature forest. Ecologically, I am...

26 February 2021 5,720 2 View

Stress/strain tensors decomposition ???

25 February 2021 6,760 1 View

Quantifying leaking DNA from bacteria - what's the secret?

Hi, all! I need to determine if my treatment causes membrane damage in bacteria (S. aureus and E. cloacae), but I cannot use a fluorescent probe such as PI and similar ones because my compound is...

24 February 2021 2,006 2 View

How the Ground Plane implantation is performed without damaging (and w/o varying the undoped nature) the top Si (8-10 nm) in FDSOI technology ?

It is a general practice to introduce Ground Plane in FDSOI devices under the BoX. The Ground Planes are introduced by Ion Implantation through Top Si layer and Buried Oxide. I wanted to know that...

24 February 2021 5,974 4 View

How to Control Whiteflies in coconut leaves?

24 February 2021 1,005 1 View