How can CRISPR-Cas9 be used to target and correct a single nucleotide polymorphism (SNP) in the human genome?

More Battula Satya Sai Naga Gayathri's questions See All

My cfDNA extraction protocol using silica beads works with plasma collected in COD SAGM bags but not with plasma collected in K2 EDTA tubes. Why so?

I am currently performing cell-free DNA (cfDNA) extraction from plasma using an in-house developed kit that employs silica-coated magnetic beads. My protocol works effectively with plasma obtained...

28 July 2024 3,559 0 View

How can i start study on warm whole theory?

To study the warm whole theory, what is the roots of the this theory and where can i start and which books will i prefer.

26 April 2024 2,788 2 View

Sample preparation for characterizing hydroxyapatite nanoparticles?

Hello all! I have synthesized hydroxyapatite nanoparticles and have to characterize them with DLS and SEM analyses. Can someone please let me know the sample preparation methodology for DLS and...

06 April 2024 7,614 5 View

Publication papers for speech recognition?

I need to require recent publication papers for speech recognition

01 April 2024 4,103 3 View

Which is the more accurate method to calculate LUMO value for a D-A system?

Which is the more accurate method to calculate LUMO value for a D-A system? Either it from absorption edge or from CV measurement on reduction side?

15 February 2024 5,635 1 View

Why are my cell-free DNA elutes translucent after cfDNA extraction using plasma samples?

I am carrying out cell-free DNA extraction using an in-house buffer system and magnetic nanoparticles. My qubit readings and tape station data look good. But upon using some plasma samples (2 out...

11 January 2024 9,426 0 View

Hello, Could someone please share with me an equation to calculate the electric field strength of an RF power supply of 250 W?

This is for my postgraduate research work.

22 December 2023 5,138 2 View

Im not able to see any protein bands.This is how our Nylon membrane looks after taking out it from semidryer.whats the problem?

we are applying 24v for 40mins.After that our nc mebrane is becoming like the image i posted, we tried to change current and timing, every time it is becoming like this.

29 October 2023 9,589 0 View

Is it possible to find the crystallite size of thin films from XRD?

It is known that W-H plot can be used to find crystallite size of nanoparticles. But, is it correct to follow the same method to find crystallite size of thin films?

10 October 2023 8,696 6 View

How much water is required to make a adobe brick?

I am going to do research in fiber reinforced adobe bricks

21 September 2023 6,328 0 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

How do you delete a duplicate pdf for the same paper on ResearchGate?

The first pdf file I uploaded had an error. So I uploaded an updated, corrected pdf of that paper with a different pdf name. I dpon't want the old copy to be download or read.

07 August 2024 9,508 1 View

Who of all the Global Scientific community will help me Prof. Dr. Yoshida make way for TPEOM, MEC ~EMC to return the atmospheric gases to the norma ?

TEP presentation caption (The Environmental Project) Re: Why should Washington’s DC, or any country government point of location think of as nowadays of as to being 'tomorrow as to come! if it...

03 August 2024 2,484 1 View

Which is more important: The human factor or the technological factor in combating climate change?

On a personal note, even though technology has attracted a lot of interest and funding to combat climate change, it is becoming more and more clear that taking care of the "Human Dimension" is...

02 August 2024 6,773 12 View

What is the ionic radius of Hydrogen anion? And how to know the exact ionic radii of the elements?

I'm trying to calculate the tolerance factors of the hydride perovskite and the value calculated by me and the reported values are contradictory. Need more clarity in using the correct ionic radii...

01 August 2024 2,614 1 View

How is the bacterial genome's high protein count verified as genuine despite 800+ contigs and good metrics (98.55%completeness, 0.68% contamination)?

Given that the bacterial genome has over 800 contigs, but its quality metrics are good, with a completeness of 98.55% and a contamination of 0.68% as assessed by CheckM, what specific validation...

01 August 2024 1,514 1 View

How to change the version of the article full-text pdf file?

How to change the displayed full article text to its corrected version? In the file on the page of the journal where I published the article, there was an error in the text, the table is...

30 July 2024 3,229 2 View

Why Powder XRD is performed after cruhsing a crystal if Single Crystal XRD analysis of the same crystal has been performed already ?

What information we can get from PXRD analysis other than from SCXRD analysis of a crystal ?

30 July 2024 6,261 4 View

Inquiry on Maximum Nucleic Acid Volume for 2.5 mL Liposome Solution?

I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...

30 July 2024 6,420 1 View

Entropy measure and QSPR modeling in Graph Theor. How to construct the table for lengthy equation?

The entropy measured of molecular graphs plays a crucial rule. The network structures in some cases are very lengthy calculations to handle. Some author avoid to construct table where as most...

30 July 2024 3,126 0 View

Sabine Strehl

These papers may help you to answer your question.

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7003079/

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8260261/

Anisa Bibi

CRISPR-Cas9 corrects an SNP by creating a DSB near the SNP site, which is then repaired using a donor DNA template with the correct nucleotide sequence. The process relies on the cell's natural HDR mechanism to incorporate the correct sequence into the genome.