How does SEA software normally number subsystems?

Dear colleagues, What is the common subsystem numbering system in SEA modelling software (i.e. VA One, AutoSEA2)? Cheers, Feng

27 February 2021 7,073 3 View

Can a Golgi-stained dendritic spines picture processed by Image J be directly used for publication?

24 February 2021 3,348 2 View

Is it possible that the phosphorylated proteins mobilize faster than the non-phosphorylated ones?

The band downshifts on Western-blot, after kinase assay treatment.

23 December 2020 7,959 3 View

How to extract dry soils such as rocks and Debris from Sentinel-2 images using Google Earth Engine?

How to extract dry soils such as rocks and Debris from Sentinel-2 images using Google Earth Engine?

17 December 2020 6,600 2 View

Species identification.Who can tell me the scientific names of these Gastropods?

Species identification. I've been doing research on communities of marine macrobenthos recently, but some of them can't be identified, which hinders my research. Who can help me identify these...

14 December 2020 8,357 3 View

What are the effects of longer exposure to siRNA (for KD)?

1) I knocked down a protein and collected cells 24 h and 72 h after transfection with Lipofectamine (to note, 5 h after transfection medium was replaced with medium with FBS). 2) KD efficiency...

08 December 2020 9,850 1 View

Why is hard to produce antibodies that are specific to a protein?

Some antibodies reveal nonspecific bands when a western blot is performed. Why is hard to find a good antibody for a protein? Antibodies produced using as the immunogen a synthetic peptide or a...

03 December 2020 208 3 View

Why different antibodies for the same protein stain different places?

I tried two antibodies and one show stain in the cytoplasm and the other one in the nucleus. Why? (Blue - dapi; red - mitochondria; green - protein of interest) And why is hard to find a good...

24 November 2020 6,612 7 View

Could bone tissue be saved in 70% EtOH after decalcification before processing?

I got the mouse tibia and femur tissue harvested, fixed with formalin 48h and decalcified with 14% EDTA 72h before paraffin embedding. This protocol works well for both HE and IHC. Now, for the...

18 October 2020 5,318 1 View

Why are G-quadruplexes a thing? Why not A, T, or C-quadruplex?

Guanine-rich DNA sequences can fold into four-stranded, noncanonical secondary structures called G-quadruplexes (G4s). G4s were initially considered a structural curiosity, but recent evidence...

27 September 2020 9,105 6 View

Anyone plz suggest relevant topic for research in image processing+Deep learning.?

I have selected brain tumor images ...but now found that already lots of research done n this topic.

03 March 2021 5,774 3 View

Which Journals would you advise to submit a Letter which briefly talks about neurosurgical treatment of neuro-oncological patient during Sars-COV-2?

Possibily not an open access journal which does not add any publishing costs once being accepted. Thank you in advance

09 February 2021 717 6 View

Why is SV40 not considered an oncogenic virus?

Hello, SV40 is not considered an oncogenic virus in this review:Oncogenic viruses and cancer Why is SV40 not considered an oncogenic virus when it produces the large T antigen, which is...

04 December 2020 8,374 5 View

Could the desmin mutation in myofibrillar myopathy be caused by an infection?

A patient with desminopathy (mutation Thr341Pro DES in a heterozygous state) with the progression of the disease has a decrease in taste and smell, immunosuppression, and an increase in IgA in the...

02 November 2020 5,886 6 View

Does anyone has experience with Dextran-FITC uptake by cancer cells?

Please share the protocol for antigen uptake assay by cancer cells. I want to use Dextran-FITC to track the endocytosis by cancer cells. If anyone has the protocol or any suggestions kindly share.

25 October 2020 469 3 View

How to Induce Ovarian Cancer?

Dear, researchers i want to introduce ovarian cancer in Swiss mice, because most of carcinogens are not organ specific, can anyone tell me which drug/carcinogens i try to introduce cancer?

12 October 2020 4,915 3 View

Ideal sample size calculation for a descriptive study ?

I wish to estimate the expression of two types of markers by immunohistochemistry in the biopsy specimens of a particular type of cancer, and correlate their expressions with clinical parameters...

20 September 2020 9,662 6 View

Cytoplasmic staining of BCL-6 in spleen tissue of treated groups rather than nuclear?

I have 3 groups of animal apart from PBS controls where i treat them under different immunosuppressive conditions. when the animals are subjected to partial or no immunosuppressive conditions, i...

31 August 2020 9,012 5 View

Dataset for medical image classification?

hello I'm a phd student my research is about medical image classification .. i need a dataset and i'm interestiin in brain image ( brain tumor) or if its not available can you provide me a dataset...

05 July 2020 7,602 6 View

Do you know any brain multi slice CT scan data set?

I need CT scan datasets which I can see different layers of brain in it.

09 June 2020 1,746 1 View