Could someone please explain the specific steps involved in integrating integrins onto an AFM tip? I couldn't find any information in the literature, so thank you.the specific steps for integrating integrins onto an AFM tip?
After COVID-19 it has seen that EFL learners technological affiliation has raised. In addition, in the post-COVID period learners started to engage AI technologies like ChatGPT while learning...
08 August 2024 8,964 4 View
How we can cite the papers from ResearchGate. I am trying to create citations for this article, Quantum Machine Learning Algorithms for Optimization Problems: Theory, Implementation, and...
08 August 2024 6,690 3 View
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
07 August 2024 6,622 1 View
I want to refine one XRD peak of my in-situ xrd but the background is never working good which ultimately fails the refinement. How to refine and adjust the background using GSAS-II
05 August 2024 5,291 2 View
Hi, i would like to simulate an absorption process in Aspen Plus. I want to use the NRTL model und would like to add some individual Henry coefficients. Is that possible and how?
05 August 2024 2,333 2 View
Hello everyone, I'm encountering an issue with my electrochemical impedance spectroscopy (EIS) measurements and would appreciate some insights. Experimental Setup: Electrodes: Gold interdigitated...
05 August 2024 3,783 2 View
AI tools like ChatGPT can enhance research work significantly when used responsibly and in conjunction with thorough human oversight.
05 August 2024 1,842 3 View
Have you ever seen a LC-MS/MS method uses both internal standards and external standards (in matrix matching purpose) but the concentrations of internal standards are outside the calibration curve...
05 August 2024 3,084 6 View
Hi everyone, I am working on brain slices for visualizing a protein in the soma and dendrites, using a fluorescence tag. However, I need a tool (not paid) for reconstruction of the whole neuron,...
04 August 2024 4,725 2 View
Citi BLOC Standard Basket Definitions: A standardized unit representing a fixed basket of construction materials, labor, and equipment costs priced in various cities. Purpose: To create a common...
04 August 2024 8,997 1 View
Did someone used specific blocking antibodies that target the adhesion molecules involved in the interaction between hematopoietic cells and MSCs (specifically antibodies against VCAM-1 or α4β1...
03 July 2023 2,524 0 View
Trying to find a fluorochrome-conjugated anti-integrin b8, the target species is mouse
03 April 2023 7,318 0 View
I wish to highlight the structural domains in the integrin receptors for which I need exact amino acid's location (start and end). Literature has mentioned the different domain names, but not...
14 March 2023 6,556 0 View
Hello, I was trying to probe for an integrin subunit that was supposed to be apparent at 100 kDa and I have two questions; 1. when I tried the first time, the bands showed up at around merely 70...
05 February 2023 1,161 17 View
Hi! It is known that different integrin has impacting macrophage's polarization, eg. ↑integrin b5, or ↓integrin b3, a3, a6 will shift the macrophage to more M2 behaving. Any more brilliant...
24 October 2022 9,581 0 View
Hi! I am looking for a summary/review of overall different integrin isoforms' affinity to different ECM ligand isoforms。 eg. integrin a7b1 has high affinity to Laminin a2, while a3b1 and a6b1 has...
23 October 2022 7,928 2 View
In the trafficking studies of active /inactive integrins, scientists usually employ conformation-specific antibodies (e.g., mAB13 for inactive integrin beta 1; HUTS-21 or 9EG7 for active beta 1)...
14 July 2022 6,679 0 View
I'm sort of new to visualization of crystal structure so please bear with me. I'm working with Chimera as my viewer as of the moment. I'm wondering how the following extended conformation of the...
03 July 2022 7,214 1 View
Hi! We recently bounce into a concept of integrin internalisation. What we previously involve is mostly integrin clustering and adhesion maturation. Seems both process are triggered by RGD...
14 March 2022 3,443 0 View
I blocked with skimmed milk, did the 1ry Abs incubation overnight at 4C. I have used two Abs (two membrane) for detection of integrin b1 using western blotting. Washed 5 times with TBST, each is...
30 November 2020 4,351 3 View