Trying to find a fluorochrome-conjugated anti-integrin b8, the target species is mouse
I want to characterize the mechanical properties of individual basalt fibers. I am having problems when choosing a test speed. If a follow ASTM C1557, I should choose a speed where the fiber...
24 March 2024 4,985 3 View
hello I hope you can help me, i am having problem to isolate a pColdI plasmid from E. coli we are using alkaline lysis but when viewing the plasmid in the gel, only a single band is...
24 October 2023 8,844 0 View
I have more papers and chapters published and available online. Is it possible to indicate also those texts at the ResearchGate? Thank you.
17 September 2023 8,444 3 View
Hi everyone! I want to compare the cell growth and lysis of two strains of E. coliBL21 by optical density (OD) measurement at 600 nm. Considering the spectrophotometer read the microplate from...
18 January 2023 8,893 2 View
I would appreciate it if anybody could point out in this image which are the ends of laticifers Thanks in advance
26 April 2022 7,096 5 View
Hi I want to perform and RDA analysis using bacterial community data and environmental data as explanatory variables. I have some variables that are percentages, others are direct measures of...
21 May 2020 9,664 0 View
Does anyone know of good sources of information/research on Western medicolegal-socioeconomic practices? I have an amazing story to tell and research to share that will be a credit to Medical...
03 May 2020 9,144 0 View
Dear scientist: I want to measure the antioxidant capacity of cosmetic creams and lotions with the DPPH and ABTS methods, but I am not sure how to prepare the samples. Will dissolving them in a...
10 February 2020 3,793 4 View
I would like to transfer the Dat-Cre transgene (B6 background) into a outbred strain (SW) of mice. How can this be done? Do I start with a single breeding pair (Dat-Cre B6 x SW) and then for every...
02 February 2020 5,021 4 View
I am designing a course on how to learn languages and I'm interested in what researchers think are the most effective language learning techniques and/or strategies.
05 January 2020 7,625 16 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much
08 August 2024 9,157 4 View
I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...
07 August 2024 505 3 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....
04 August 2024 762 1 View
I need to lyse and homogenise mouse tissues. So far I have experimented with liver and lung tissue, using matrix D. The liver was completely lysed with 6 repetitions of the standard program, but...
31 July 2024 9,186 0 View
I need to Postfix mice brains. I slice the brains using microtome at 40 microns. These are Postnatal days 7, 11, 14, and 21. I am using half the brain for other analysis, so I need to fresh freeze...
31 July 2024 1,123 4 View
We have a lateral flow test on Sartorius 140 NC. The conjugate is gold-monoclonal antibody. Using different control lines (GAM, protein A, protein G) we get a very strong leading edge with reduced...
31 July 2024 4,510 3 View