Hi Selvaraju

The best estimator of holocellulose would be assessing the lignin content on the extractive-free wood (plus the soluble lignin if hardwood is in question, for softwoods no need), Holocellulose would be 100%- lignin content %. If you are interested in extracting holocellulose it is a different procedure.

Please provide me a procedure for holocellulose extraction.

Attached the copy of pthe holocelluse according to  Browning BL (1967). Methods of Wood Chemistry. Please note there are no method capable of extracting a pure holocellulose fraction. All available procedures have two problems 1) incomplete lignin removal and 2) loss of carbohydrates during isolation. That's why I mentioned earlier that the best way to determine holocellulose is performing the lignin analysis.

Another method to determine Holocellulose content:

Holocellulose is the total carbohydrate fraction (cellulose and hemicellulose ) of the raw material and can be estimated according to Wise et al., 1946 as follows :

            The raw material is ground in a laboratory Condux mill to pass a screen of 0.3 mm opening diameter and then extracted with an alcohol - benzene mixture (1:1) for 6 hours. 5 gram of the raw material (exactly weighed) are suspended in 150 ml water at 75°C and the solution is stirred mechanically. 10 drops of glacial acetic acid and 1.5 g sodium chlorite are added and the reaction has been allowed to proceed under vigorous stirring. After 1 hour the same amount of reagents are added and this process is repeated until the total reaction time was 4 hours . The solution is then cooled, filtered and washed with distilled water until free of acid, then with acetone and left to dry. The holocellulose is then estimated gravimetrically .

Wise, L. E.; Murphy, M. and D'Addieco, A. A. : Paper Trade J. 122 (2), 35 (1946).

Thank you all for your information.

Dear El Barbary Hassan

Thank you very much also for the reference, would you be abble to get a pdf?

Kind rgards

josé

Dear experts,

Do you think that it is possible to use NaClO instead of NaClO2?

ash content must be subtracted from dried biomass

The below mentioned researcher has many articles involving determination of holocellulose, cellulose, hemicellulose, and lignin for different types of biomass. Look specially at the older articles. Happy new year.

https://www.researchgate.net/profile/Yehia_Fahmy

What kind of filter do you recommend? Whatman or glass disk?

Hello

Kindly look at link below

https://www.academia.edu/17525278/METHODS_FOR_DETERMINATION_OF_CHEMICAL_COMPOSITION_OF_PLANT_BIOMASS

Good Luck

Hi,

This reference can help you:

https://www.sciencedirect.com/book/9780128129920/biomass-gasification-pyrolysis-and-torrefaction

Hi dear Byron Lapo

Do you think that it is possible to use NaClO instead of NaClO2?

I think NaCLO can be used, because I did it.

best regards

alguien puede facilitarme este articulo:

Wise, L. E.; Murphy, M. and D'Addieco, A. A. : Paper Trade J. 122 (2), 35 (1946).

muchas gracias

yey you can use NaClO instead of NaClO2