How can a person see dots and positive result in dot blot but see no bands at western blot test?

Dear Scientists After protein expression, I did periplasmic and total protein extractions. I did the dot blot test to see if my proteins have been expressed. well, The dot blot result was perfect...

31 December 2018 6,167 7 View

I have problems with my SDS-PAGE results... I cant figure out the problem with my work. Is something wrong with my SDS run or protein samples?

Dear Scientists I ran my SDS-PAGE with 37.5 microliter protein + 12.5 microliter sample solvent in every well and with stacking gel with pH=6.8 and separating gel with pH=8.8 with 100 voltage for...

31 December 2018 509 3 View

Why does a protein protein complex fall apart during simulation with gromacs?

during a 200 ns simulation of a protein complex in water in the 150th nanosecond they fall apart. I have retrieved the complex from PDB. I wonder whether someone can help me with that should I...

09 October 2018 9,737 6 View

What is the best way to calculate protein-protein binding affinity and protein-protein binding free energy using gromacs?

Dear researchers I wonder whether you could help me with that question.

07 August 2018 1,708 1 View

should i remove the His Tag from my protein for surface plasmon resonance?

I am expressing my protein in E.coli expression system and to purify it I am gonna use His tag. After this i am going to use a method to measure the binding affinity between this protein and some...

31 December 2017 6,538 3 View

Will E.coli be able to express this protein?

Dear scientists I do have a mammalian protein which i want to express in E.coli.as we know one of the important factors in protein expression is the mRNA secondary structure. It's better for the...

31 December 2017 7,116 2 View

Hi.In simulating with gromacs should i simulate both monomers of a homodimer or one is enough?

I am using gromacs and the protein I use is a homodimer and both dimers bind from the same region to receptor, to calculate the free energy of binding do i need to use the dimer as input or the...

08 September 2017 179 4 View

What are the odds of 2 different binding energy calculation softwares (say foldx and gromacs) having the same result?

08 September 2017 8,631 3 View

Hi.I wanna calculate the protein-protein binding free enenry with gromacs by MMPBSA where can i find a thorough step by step protocol for that?

I have found tons of complete step by step protocols for Amber.But i should do this with gromacs...but I can't for the life of me find a step by step (from A to Z ) tutorial for that...in...

07 August 2017 2,950 1 View

HI.How can i find a through protocol of process of the manufacture of an approved medicine?

Do the pharmaceutical companies publish a through protocol of the drug they sell?if yes how can we have access to them?

06 July 2017 6,058 5 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

Which is suitable for use with Python? MySQL or SQL Server?

Which is suitable for use with Python? MySQL or SQL Server? What is your suggestion?

01 March 2021 3,422 3 View

Can Virtual Servers (remote dekstop manager) run heavy ANSYS Fluent CFD simulation ?

I have a virtual machine. My host Pc specs are not that good thats why i went for virtual servers. so basically my host pc has 8gb of ram with nvidia quadro k2200 4Gb DDR5 GPU ,processor intel...

01 March 2021 8,415 1 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

How to plot the probability paper of Gumbel distribution using R programing?

In R-studio, there are many commands of Gumbel package. Arguments are also different. I`m asking about the alpha parameter of the Copula which must be greater than 1. If this is the one used to...

25 February 2021 5,229 3 View

Bayesian decision trees and bayesian regression trees in Python and/or R?

I am looking for implementation of bayesian decision trees and bayesian regression trees in Python and/or R.. Do you know if the existing libraries support this

23 February 2021 6,689 3 View

Are there packages (R or Python) for simple models (bucket?) to assess soil water content?

Hi everyone. I am researching droguht effects on soil water drought. I am looking for already implemented functions in R or Python to estimate soil water content from precipitation,...

21 February 2021 2,084 1 View

ANCOVA controlling baseline in RCT - how to perform power analysis and sample size estimation?

Hi, I have a simple question. I am hoping to perform a power analysis/sample size estimation for an RCT. We will be controlling for baseline symptoms, and using post-treatment or change scores as...

19 February 2021 2,648 23 View

MVN, how to conciliate contradictory results from Multivariate normality and Multivariate outliers test?

Dear colleagues, I'm working with Path analyses in lavaan and MVN packages. There are some results that for me are confusing and features from the MVN that I do not know how to set. My dataset is...

18 February 2021 190 3 View

Would you please recommend a function in R software that could be used with FUNGuild output to merge different rows that have similar guilds?

Dear colleagues, I have an output of FUNGuild tool (ASVs abundance in different samples and assigned to guilds) and would like to merge the ASVs that had similar ecological guilds using R...

16 February 2021 4,292 7 View