Hello, I would like to perform high-throughput measurements with our LC-MS/MS system. We have a Vanquish Neo (VN-S10) and I would like to inject my sample directly from the 96 well plate (500 µL). With our somewhat older vanquish Neo, injection from the well plate with a sealing mat (Eppendorf) works without any problems. With the newer vanquish Neo, the error message “Septum too strong/tight for bottom detection” sometimes appears. The method settings of the two LCs are identical, the error only occurs with the newer one. I would then deactivate the bottom detection option, does anyone have experience of this issue or an offset setting to avoid damaging the needle? Thanks.