How to purify cycle sequencing PCR reaction?

i am purifying cycle sequencing PCR reaction. i have tried multiple methods given in BDT manual (provided with the instrument) .everytime i used 96 well reaction plate for this purpose. 7 wells...

14 February 2021 8,534 2 View

Does any one have experience with Autoclavable cell culture media (RPMI 1640/DMEM)? Does it work well?

12 February 2021 2,958 1 View

What are the reasons of asymmetrical bilateral knee Osteoarthritis (OA)?

Primary osteoarthritis - is degenerative joint disease without any apparent underlying cause then what are the reasons of asymmetrical bilateral Knee OA?

09 January 2021 7,524 8 View

Zero normal flux boundary condition for shallow water equations?

I am looking for any book/article reference about the mathematical description of zero normal flux boundary condition for shallow water equations. My concern is that for a near-shore case how it...

16 December 2020 9,383 8 View

How to fix the fungus for SEM imaging?

I want to perform the SEM of candida sp. and i want to know that the fixation for the bacteria and fungus are different or same?

10 December 2020 6,169 3 View

How to adapt a mature Questionnaire?

I am looking forward to seek some help regarding adaptation of mature questionnaires. I am new to quantitative studies and wants to use some demographic in my questionnaire, is it necessary to add...

08 November 2020 7,146 5 View

Primer degradation.....what could be the possible reasons for not having PCR results.????

HI! whenever I start doing PCR with a new primer pair that is lyophilized.1st I make 100pmol solution then 10pmole dilution of it as a working solution. I got good results with this freshly made...

01 September 2020 2,114 15 View

Which are the best open source plotting software?

Dear friends, Please suggest few good graph plotting software that are freely available. If it has an contour plotting option, it will be an added advantage. Origin is a paid software which is...

06 July 2020 9,106 15 View

How can I import a user subroutine into Abaqus component in Simulia Isight?

For optimization my material parameter I use Isight software. I have an input file that simulates the material model via UMAT subroutine. How can I import this inp. file in the Abaqus component of...

14 May 2020 3,552 3 View

Name the hydrological models, which can simulate ice dynamics?

Please explain their principles briefly with references and flowchart. What are their advantages and limitations?

12 April 2020 2,718 1 View

How to calculate the crystallinity of cellulose film?

27 February 2021 2,753 4 View

What is the optimal method to prepare a controlled porosity osmotic pump (CPOP) tablet for scanning electron microscopy?

In order to assess the shape and size of pores formed by dissolving the pore former in the semipermeable membrane structure, SEM images are needed. Cellulose acetate was used in the coating...

15 February 2021 6,135 3 View

Is carboxymethyl cellulose a better electrolyte host polymer than methyl cellulose and cellulose acetate?

I want to prepare a cellulose-based biopolymer solid electrolyte by solution casting technique. However, choosing the right cellulose derivative has been my major challenge. Among the many factors...

30 January 2021 5,355 3 View

What is the mechanism or interaction between Fe3O4 magnetic nanoparticles and biochar?

Lately there are many studies on biochar incorporated/loaded/coated with Fe3O4 magnetic nanoparticles. But I havent found any paper that describes the interaction or bonding between the two. How...

20 January 2021 3,162 4 View

After cellulose is regenerated in water, a cellulose hydrogel is formed. I want to know if there is crystallinity inside the the hydrogel?

After cellulose is dissolved and regenerated in water, a regenerated cellulose hydrogel is formed. I want to know if there is crystallinity inside the hydrogel, or it will not crystallize until it...

04 January 2021 7,466 4 View

How to calculate "POWER LAW" in rheology from a GRAPH of shear rate vs viscosity for cellulose nanofibrils suspensions?

How to calculate "POWER LAW" in rheology from a GRAPH of shear rate vs viscosity for cellulose nanofibrils suspensions?

02 January 2021 6,816 3 View

Crystallinity index measurement of Cellulose nanocrystals ??

How to find the crystallinity index of cellulose nanocrystals using XRD peaks. Can anyone suggest the most appropriate method of finding CI

28 December 2020 7,809 4 View

What is the best solvent and dose of Alloxan to induce diabetes in mice ?

Saline water or citrate buffer or carboxymethyl cellulose sodium salt

13 December 2020 4,110 1 View

What is the glucose content in Cellic CTec2?

Hi, I've determined the glucose content in Cellic CTec2 enzyme blend and it is 12-14 mg per 100 mg of enzyme blend. Could anyone share with me your results so I can compare it with mine?

08 December 2020 6,312 3 View

Reason for growth of fungus/mould in hydrothermal liquifaction filtered product of cellulose-based materials?

Performed hydrothermal liquefaction of cellulose powder, at about 200 degrees for 10 16 hours. After simple filtration, I stored filtered product at room temperature for 4-6 days, there is the...

03 December 2020 7,327 3 View