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Transwell migration assay - cells dying !?

Hi all, using a transwell assay to test migratory function of cytokines, Costar transwells 5 micrometer, 24 well, according to publications and protocols 100 ul in top compartment with 1x10*6...

09 October 2011 4,678 2 View

MSP stadardization problem

Read 1 answer by scientists to the question asked by Axel Hueber on Oct 8, 2009

09 October 2009 6,627 0 View

protein science

Read 2 answers by scientists to the question asked by Axel Hueber on Oct 8, 2009

09 October 2009 7,156 1 View

Caspase 3 - Please Help!

Read 4 answers by scientists to the question asked by Axel Hueber on Sep 18, 2009

08 September 2009 1,153 2 View

nonspecific bands in CO-IP

Read 2 answers by scientists with 1 recommendation from their colleagues to the question asked by Axel Hueber on Sep 18, 2009

08 September 2009 6,525 0 View

SINGALING PATHWAY IN A CELL

Read 1 answer by scientists with 1 recommendation from their colleagues to the question asked by Axel Hueber on Aug 1, 2009

07 August 2009 3,593 0 View

i need help w HMEC culturing

i'm culturing my HMEC in gelatin -coated flasks (by me) using MCDB medium and EGF, heparin and FBS (10%) and the cells did not attach... what...

07 August 2009 6,047 0 View

copy number determination: sauthern blott vs real time pcr

Read 2 answers by scientists to the question asked by Axel Hueber on Aug 25, 2009

07 August 2009 156 0 View

Sequencespace

Read 1 answer by scientists to the question asked by Axel Hueber on Aug 25, 2009

07 August 2009 1,030 0 View

why sw480 cells do not growth up ???

Read 1 answer by scientists to the question asked by Axel Hueber on Aug 25, 2009

07 August 2009 4,762 0 View

How to compare 2 samples recruited with different sampling methods?

If I want to compare characteristics of 2 groups of patients, one group is recruited from a random sample of the general public while another group is from a sample with a fixed gender (male to...

26 February 2021 3,526 3 View

Do you consider undergoing a structure literature review / Meta analysis and getting numerical findings sort of quantitative method?

I am looking for a classification of the research methods included in literature review papers or Meta analysis ones .

22 February 2021 1,131 4 View

Can I do two kinds of mixed method in explanatory research?

Dear distinguish professors and scholars, I have a research in measuring two kinds: think tank capacity and research impact on policy. Can I do two kinds of mixed methods in order to find, "Is...

20 February 2021 6,897 1 View

How to analyse a (perceptual) study using differing/comparable sets of stimuli?

I plan on running an online perceptual experiment that has, let's say, 100 unique video stimuli altogether. Since these are too many to be rated by every participants, I would like to present the...

15 February 2021 7,444 2 View

How to analyse a split-plot design in stata ?

I have run an split-plot design experiment evaluating the efficacy of a treatment (with 3 whole plots and 4 sub-plots), and I am planning to analyse the collected data in Stata. However, I do...

14 February 2021 7,800 7 View

Can I use a likert survey for criterion sampling in a qualitative study or would that make it mixed methods?

I am doing a phenomenographic study on the reading experiences of avid v. Neutral v. Non-Readers. If I use a likert survey to criterion sample for participants, does that make it a mixed method...

09 February 2021 8,594 3 View

How to create the context-mechanism-outcome configuration (CMOc) in the realist methodology?

In the realist methodology (especially by Raymond Pawson et al), the CMOc is a common structure to explain what works, for whom, in what circumstances, and why? I'm always asked about the...

08 February 2021 2,792 1 View

Recommendations for sample size in visual qualitative research (using children's drawings)?

Hi everyone! Asking for a little help, if I may. I am working on a research project, where I aim to analyse children's drawings about Covid-19. I am fairly new to using visual data in qualitative...

07 February 2021 9,530 3 View

How do I create a categorical variable, level of exposure, and a dummy dichotomous, yes-no, variable for the Harvard Trauma Questionnaire Part 1?

I am using the Harvard Trauma Questionnaire (HTQ) Trauma Events scale (original version) that has 17 items (17 different traumatic events) with 4 response options, indicating 4 levels of exposure:...

04 February 2021 1,509 3 View

How to report path analysis / SEM with Yuan-Bentler correction from R output?

Dear all, I am currently writing my master thesis where I am analysizing my data using path analysis and SEM in R (lavaan package). Now that I like to write up my results, I am struggling with...

04 February 2021 8,993 2 View

Muhammad Aslam buzdar

i am cloning one enzyme of yeast when i do pcr i get band of my interest but very thin which is not retriveable or may not be use for transformation.ialways i am using standared methods i have also change concentration of DNA but same result.plz suggest me any thing.

one thing more how should i published my this problem on research gate i did get place where i should i write it.

Aslam

Andrea Cossu

What's the gene's lenght?

What's your extension time in pcr at 72°C?