Since I don't have enough space in my Flow panel I have to choose one of these activation marker (CD38 or HLA-DR). I wonder which one is more important?
Activation marker for which type of cells? If you are working with DC or monocytes HLA-DR is the way to go.
Actually, you can also use HLA-DR as an activation marker for T-cells. Jean Kwun is right that T-cells express low levels of MHC-class II in resting state, but upon activation they highly express HLA-DR. But I think CD25 and CD69 are more commonly used. I understand that your FACS-panel is already pretty full, otherwise you might want to consider CD137 or CD154 to look at antigen-specific activation of CD8 or CD4 T-cells respectively. These markers peak 24h after stimulation.
Thanks Marleen, I thought CD69 is mostly early activation marker and also a marker for tissue resident cells. also CD25 are usually highly expressed on B-Cells right? in our department every one use CD38 and HLA-DR as activation marker. However, I am not sure which one is better if I just want to choose one of them.
Hi Avid, I have no experience with CD38. Also, I don't know at what time point after stimulation you want to measure (which can influence the decision for a specific marker, as some are earlier upregulated than others).
I found the following paper that compared CD38, CD69 and CD25 upregulation on T-cells after diverse stimulations: http://www.jleukbio.org/content/77/4/513.full
Sorry to say so, but from these data I'd rather go for CD25 or CD69 :D
I would recommend you do a small pilot experiment and compare HLA-DR and CD38 upregulation after the stimulation you intend to use?
Thanks for your help Marleen. really appreciated. Well I don't want to stimulate cells in-vitro I want to know their activation status as I isolated them from PBMC or peripheral tissue. but maybe I can do the pilot to compare HLA-DR with CD38!
Isn't CD38 a marker of immunosuprression and exhaustion? I read a paper stating that CD38+ T cells are immunosuppressive and can be found in inflamed tumors. I
It will be good to know how others have evaluated CD38+ T cells and CD38+HLA-DR+ CD8 as well as CD4 T cells
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