Hi, does anyone know of a method to measure blood oxalate levels accurately from samples stored at -20 or -80 for months to years? Is it possible?
I know the Mayo Clinic lab uses an ion chromatography method that requires immediate acidification and centrifugation of samples and samples to remain on ice. Sigma makes a research kit that uses spectrophomometry.
Any one familiar with this test or others that i could use on old frozen blood samples.
Thanks!
stored samples. The methods used for determination of oxalate in blood are reviewed, and the advantages and disadvantages of the two basic approaches-direct methods and in vivo isotope-dilution techniques-are compared. Possible reasons for the previous discrepancies between direct and isotopic methods are discussed, as are the effects of protein binding, sample handling, and storage conditions on oxalate values in plasma. Necessary precautions for obtaining reproducible results are presented. We recommend and critically review several direct methods, and describe the application of a direct method for oxalate determination in some other biological fluids. I think the following below links may help you in your analysis:
http://citeseerx.ist.psu.edu/viewdoc/download?doi=10.1.1.531.7877&rep=rep1&type=pdf
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4485608/
Thanks
Dear
If these are in ppm level you can very well use HPLC method , anion exchange column, conductivity or UV detector. If these in ppb level you may need t go for LC/MS technique which is capable of quantifying down below 50 ppb level
Regards
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