Hi. I want to do qPCR to evaluate SREBP1 mRNA levels in VERO cells. Can any of you suggest me a primer pair that will work in this cell line?

05 May 2016 1 6K Report

qPCR will be done with SybrGreen

Pubmed or Google Scholar will help you find publications that have done real time for your target. Otherwise IDT and Roche have great resources for primer design:

http://www.idtdna.com/pages/scitools

https://lifescience.roche.com/webapp/wcs/stores/servlet/CategoryDisplay?tab=Assay+Design+Center&identifier=Universal+Probe+Library&langId=-1

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