How to solve a problem in analysis of colistin sulphate using HPLC-UV?

Hi everybody, I am setting up a method for analysis of colistin sulphate by HPLC-UV. I tried different previously-published methods but the results were not acceptable (long run time, bad...

16 January 2021 5,911 3 View

Does hyaluronic acid aggregate in the pH range of 3-4?

Dear researchers and Scholars Is it possible for aqueous hyaluronic acid solution to aggregate when entering to the colloidal nanosolution with pH about 3-4 ? I want to add hyaluronic acid to coat...

09 September 2020 9,688 3 View

How to get stable polymeric micelle?

03 July 2020 8,476 2 View

Why absorbance of dye solution increases after long irradiation time?

I have observed that the absorbance is increasing at long irradiation time during photocatalytic experiment for a perovskite material .Even I completely separate the catalyst particles with the...

13 December 2019 3,911 5 View

Has anyone ever used FCN (deeplearning) for one dimensional data in structural damage detection?

I'm working on deep learning in structural damage detection on bridge by signal (that is one dimensional data) . for that i want to get some information about Fully Convolutional Networks (FCN) in...

13 November 2019 9,347 3 View

Connected component label in matlab?

I want to connect these labels .can I just connect these without converting it to bw ? and I need help in the algorithm of connecting these labels in matlab.Thank you

19 July 2019 7,777 5 View

Parsing of building facade in image processing?

Hi, I am trying to to have a segmentation of building facade just like these two pictures that I have sent.I have tried watershed algorithm but unfortunately it did not work. I mean I could not...

20 June 2019 9,511 3 View

Is degenerate primers appropriate for real time pcr?

hi... can I use degenerate primers for real-time? I have some genes related to the enzyme in the root but these genes are gene families .and I don"t know which gene controlled my desire enzyme,...

20 December 2018 3,452 2 View

Why do i have band in negative control in ITS primer?

I look for variation in strain bacterium and I want to do sequencing but I have a band in negative control that same in my strain bacterium first time I noticed band in my negative control, I...

02 December 2018 5,889 10 View

How to generate retroviral producer cell line using big construct?

Hi there, I want to generate retroviral producer cell line using Phoenix eco cells for transfection and E86 cells for transduction. My construct is about 9.2 kb but the insert part (from 3' of...

11 June 2018 6,842 6 View

Chlorinated organic formation in odor control scrubbers using hypochlorite?

I'm involved in a study of odor control technologies for municipal wastewater treatment plant. One of the control options involves a chemical 2-stage (acid/alkaline) packed bed scrubber. The...

03 March 2021 3,661 2 View

What solvent can I used for Paal Knorr reaction between 6-amino hexanoic acid and 2.5 hexanedione?

I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...

02 March 2021 4,443 2 View

How to regulate SSCP temperature in simple PAGE?

I'd like to perform single-strand conformation polymorphism (SSCP) in my thesis, however I cannot control the temperature of the vertical PAGE since we are using the conventional tanks. Is there a...

02 March 2021 9,157 1 View

Ester cleavage conditions?

Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...

01 March 2021 8,766 3 View

How to remove DCU and HOBT from my reaction?

I am doing a acid and a chiral amino alcohol reaction using DCC and HOBT in DCM or THF? Can anyone suggest me how to remove DCU and HOBT from a reaction mixture??

28 February 2021 2,153 1 View

Can polymer concentrations be calculated based on the number average molecular weight?

Hello, I spin-cast two different polymers onto wafers and measure their height as a function of initial concentration. For polymer A, I have the weight-average molecular weight (Mw) as well as...

28 February 2021 7,882 2 View

What is the density for pva electrospining?

I'm Juan Carlos, a high school student who is working with electropinning, I made an electropinning machine with a 21g needle and a 30kv high voltage source and the distance between my needle is...

28 February 2021 298 3 View

What is the most common number of epitopes in an multi-epitope Vaccine?

hi everyone, I'm interested in the field of vaccine design specifically multi-epitope cancer vaccines , I want to know if there is a minimum and maximum number of epitopes we could use in a...

27 February 2021 3,882 3 View

No title

I am trying to find how to know the density needed for a pva electrospinning in a 0.6mm neddle, the electrospinning will be from top to bottom and the problem I really have is the solution because...

25 February 2021 2,615 3 View

How to increase solubility of Poly acrylic acid in Dioxane solvent?

I am working with Polyacrylic acid (100.000Da) and instead of water, I need to use another solvent (dioxane) to dissolve PAA. However, because of the high molecular weight, it's quite hard to...

25 February 2021 2,225 5 View