Expression cloning by using cDNA,do I need to modify the cDNA first?

I am going to have a expression cloning of mammalian gene by using shuttle plasmid to transforming the E.coli However I don't know I should only inserting the Coding sequence ,or I can...

28 February 2021 5,440 3 View

Schrodinger Desmond runs fine on one GPU, but crashes on other GPU?

Hi, We are having issues using Desmond on our workstation. Simulations run fine on one of our gpus ("Gpu0") but crash consistently on the other gpu ("gpu1" - which is also set up as the display...

01 February 2021 5,866 3 View

How does climate change affect birds (shorebirds)?

I m recently investigating the impact of climate change on shorebird population trends, I would like to know what is the impact of fluctuations in variables such as sea surface temperature, global...

10 November 2020 9,293 15 View

Can I use the EPIC-Oxford FFQ for free?

Designing my PhD project and need to use an FFQ, but we don't have any funding for this part of the project.

09 November 2020 4,541 1 View

Is there a survey tool (similar to REDCap) through which we can send an anonymous survey and restrict respondents to one survey each?

I am working on a research survey where I need to send it out to a group of users and track in (anonymous) responses. I need to restrict users to completing the survey only once. I do not think...

06 August 2020 918 2 View

Western Blot Transfer Band Smearing?

Anyone have any insight? It's becoming a recurring issue. I make fresh transfer buffer each time.

29 July 2020 9,331 9 View

How many users can i put in the same vsat broadband plan?

sorry if i wasn't able to explain very well for example, i am in a country that doesn't have satellite internet service, and would like to be the first provider for this service. lets say i have a...

05 July 2020 4,637 1 View

Failed gateway cloning?

Hi everyone, I am having a few doubts about gateway cloning. We are using gateway cloning for protein expression using pDEST15 as our destination vector. I attached a picture of the pDEST15...

10 March 2020 7,472 2 View

Inrush control when Transformers are Energized at Solar Renewable is best achieved how?

Increasing use of solar renewable resources or farms on weak remote locations are causing significant power quality and voltage notch issues with connecting utilities. High penetration of these...

17 August 2019 7,097 2 View

Glass microballoons from sodium silicate solution?

I once produced microballoons from sodium silicate solution (water glass). does anyone have experience in manufacturing these, are articles you could point me in the direction of? thanks

01 July 2019 8,764 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

How UAV or drone can be beneficial to better estimate crop yield?

As we know, crop yield estimation has been done traditionally with farmer's expert knowledge, deductive sampling in representative areas, and remote sensing with satellite imagery. Farmer's Expert...

01 March 2021 3,468 3 View

Analysis of CRD two factor using R studio ?

Hello everyone. I have conducted the research on post harvest longevity of tomato crop using plastic packaging and different doses of chemicals as two different factors. The design of experiment I...

28 February 2021 8,189 3 View

Where i will get the Desmodium and Napier grass seeds?

26 February 2021 975 4 View

What are the LAB methodologies (procedures, equipment etc) for CO2 enrichment experiments to study crop responses?

I am doing a proposal for a research entitled 'GRAIN YIELD AND QUALITY RESPONSES OF WHEAT GENOTYPES UNDER CO2 ENRICHMENT AND EXTREME EVENTS' I will be using climate chamber and I would like to...

18 February 2021 6,494 4 View

Can bio-decomposer be a successful alternative to reduce residue burning?

To maintain the proper crop plan most of the times there is not enough time to make the field ready for the next crop. In this particular context , can bio-decompser be a solution? What about...

16 February 2021 7,068 24 View

How much costs direct reduction of nitrogen on farmland in terms of lower crop yield?

Dear research fellows, I wonder if someone knows how much direct reduction of nitrogen on farmland costs in terms of lower crop yield expressed as EUR/kg nitrogen (or similar)? I understand that...

15 February 2021 1,323 3 View

What is the paradoxical effect of VINASSE in terms of low pH (<4) and high EC (>50dS/m)? What is its impact on soil properties and crop growth?

What is the paradoxical effect of VINASSE in terms of low pH (50dS/m)? What is its impact on soil properties and crop growth?

15 February 2021 3,142 6 View

How to obtain regions around the person bounding boxes(excluded the detected people in scene)?

Hi all. I have a set of coordinates of bounding boxes for AVA Dataset. This dataset is used in action recognition. My goal is to get the areas around the people (people are inside boxes) and do...

13 February 2021 2,538 2 View

What can be use to mop up heavy metals from vegetables or soils in quarry mining site?

It has been established that quarry mining site polluted and contaminated it's environment with heavy metals, these affects the crops and vegetables planted in the area. The possible solutions to...

12 February 2021 1,155 5 View