I am trying to extract the pigments from the skin, muscle and scales using 3N Methanolic-HCl (Sigma). The first and second time I tried I got excellent results from the skin, a bright green pigment (attached a photo showing the colour). However, in the following attempts of pigment extraction I didn't get the same colour, but a more yellow-orange pigment instead. I have tried several times following the same protocol. Do you have any ideas of why this is happening?

Regards

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