Why would you bother? Standard growth curve of a bacterium can be done by measuring the cultures optical density (OD) at a 600nm wavelength using a spectrophotometer or similar instrument at regular time-points. This is much simpler than your suggested method.
You could use Hoescht stains but you would need a flow cytometer (much more expensive) to count the numbers of cells that are present in suspension. Again. Wildly unnecessary as this can be done without dye. The only benefit this has is to tell you the number of alive versus dead cells by measuring ration of free dye to absorbed dye.
While the OD600 mentioned above is certainly cheaper (and I'd recommend it assuming you don't have a specific reason not to).
That said, you asked...
Plate a known number (or proportion) of bacterial cells on (eg) a 96 well plate in dilution series. Load up with Hoechst take readings on a flourescent plate reader. Build a standard curve. Compare your target to the curve.
Sometimes bacteria make clumps and show wrong high OD. So I was thinking to try this strain to get growth kinetics.
Thank You @Colman O' Cathail and Matthew Sobo
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