sgRNA after annealing product concentration is good after transformation self ligated colonies were seen, product size is 20bp unable to screen through Restriction and also through agarose gel
step 14 of this protocol:
https://benchling.com/protocols/5DmqRd/crispr-mediated-gene-disruption-in-ch12f3-2-cells/sbs
https://www.researchgate.net/topic/sgRNA
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Thank u for the needful information provided I will consider the above mentioned protocols
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