I isolated some bacterial species associated with periodontal disease and I need to detect virulence factors
Hi
I think when you isolate bacteria you can not use HPLC method, you can just do PCR.
You can use HPLC in sample saliva and you have to had standard for comparing.
A rather vague question, as virulence factors associated with particular infections might range from toxins to metabolic pathways.
In my openion you should inculated your isolate in biomaterial as meat or plant and send it for HPLC
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