Hi, any body can help with a clear detailed procedure for the determination of the binding constants by using UV-Vis. spectroscopy?

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Teodoro S Kaufman

use job's plot

Asim A. Balakit

Thanks a lot Teodoro S Kaufman, any more details about the practical approach of this method?!

Udaya Jayasundara

You may read my recent publication partly based on determination of K .

Further, you may read the abstract

http://pubs.rsc.org/en/content/articlelanding/2011/cs/c0cs00062k#!divAbstract

Thanks Udaya

Could you please refer to the link of your article?!