How to determine ploidy of different yeasts(Rhodotorula,Rhodosporidum,Candida,Saccharomyces,Lipomyces....genus) by arresting them in G1 phase of cell cycle.
Hi all, I have generated two gene expression curves by measuring bioluminescence every 10 mins for 12 hrs. These curves tend to overlap at at some time points, while a difference can be seen at...
02 February 2021 2,613 7 View
The silver nanoparticles, which are solid form and I want to study the XRD pattern of this nanoparticles by Nuclear Magnetic Resonance (NMR), so I asked which solvent are used for dissolve of...
13 January 2021 3,434 4 View
Can anyone help me with the FEM coding for Buckling of composite plates using the FSDT or HSDT - Source or the helping material (textbook, research paper etc) - Geometric stiffness matrix...
03 January 2021 3,059 3 View
I am trying to simulate subcooled boiling flow through an annulus using Fluent. Is it possible to get the values of interfacial heat transfer coefficients from either FLUENT report or CFD post?...
31 October 2020 3,599 3 View
Can someone help me with different watershed management practices for hilly areas ? Requirement is to have enough water for crop cultivation. I am looking at the hilly areas, any proof of concept...
05 October 2020 5,641 15 View
Due to COVID- 19, pupil teacher of teacher training programs are doing school internship in online mode which they are completely opposed to. What do you think as to it will adversely affect their...
09 August 2020 756 20 View
I need to run simulations running 100s of nodes in a simulated network and have to do that many times over. I could use Google Cloud Platform's $1000 provided for researchers doing PhD but I...
07 August 2020 4,537 3 View
Hi Everyone, Entecavir 0.5 mg is usually given for patients with chronic hepatitis B to keep the virus under control and as well Vaccine is available for restricting the virus. If Entecavir 0.5...
22 June 2020 5,661 3 View
So far, RT-PCR testing has been the frontline response to the COVID-19. PCR testing is considered as the gold standard for detecting COVID-19 and was designed by WHO, soon after the virus was...
21 April 2020 1,139 16 View
Recent studies from Oklahoma Medical Research Foundation, University of Queensland Centre for Clinical Research, and Qingdao Municipal Hospital identified that Chloroquine phosphate, a drug used...
17 March 2020 6,929 94 View
Microorganisms of interest include; E.coli; Salmonella typhimurium; Pseudomonas aeroginosa; Streptoccocus pneumoniae; Staphyloccocus aureus and Candida albicans. Locations closer to Port...
18 February 2021 6,500 3 View
Hi everyone, We are doing western blot with yeast protein extracts. We are applying a wet transfer method using Tris-Gly as a transfer buffer (with 10% methanol) and running it with constant...
25 January 2021 4,228 6 View
We are conducting a measurement of Ribonucleic acid from baker's yeast (S. cerevisiae) (Sigma-Aldrich R6750) concentration using Eon Biotek Microplate reader. We dilute the RNA in various...
24 January 2021 9,830 3 View
I have tried to use TTC agar overlay method for a few times to identify respiratory-deficient yeast which were affected by EthBr. The problem is that cells which should have stayed white also have...
22 January 2021 585 10 View
Good day. I would like to extract total RNA using a kit from MRSA and candida for gene expression. i tried sub-culturing standardized inoculum (50ul) with 500ul of my plant extract sub-MIC and...
23 December 2020 5,076 3 View
I want to perform the SEM of candida sp. and i want to know that the fixation for the bacteria and fungus are different or same?
10 December 2020 6,169 3 View
Besides the usual autoclaving etc. We have had several incidences of yeast contamination (suspected S. cerevisiae) after a few days of fermentation, even though all media is properly sterilized...
15 November 2020 9,603 9 View
I am working with yeast saccharomyces cerevisiae -BY4741- strain. I tagged the Atg8 with GFP in this background and whenever I am performing western blot I get a free GFP signal for my WT which...
15 November 2020 8,556 3 View
I am also a little confused as to what happens if you were to take mid-log cells (for examples' sake say OD600 ~0.3-0.8) and dilute to below 0.3. Do they slow their growth rate in a quorum...
12 November 2020 6,327 1 View
The strain is Candida tropicalis. It has been stored in 4 degree refrigerator for a year. Now we want to see if it is possible to revive the strain and make it function again. I tried to culture...
10 November 2020 5,650 9 View