Can you elaborate your question?

whetHer you are looking for process impurities or degradation impurities or any major known impurities?

Sorry, I am not getting your question.

Specify your question.

hello all respected sir, i want to know the how the sample is loded manually and how the impurity is to be isolate on hptlc manually

Preparative HPLC is comonaly used for isolation for impurities , in prep we manuely inject sample by syrine through injector loop, peak is saperated and required peak is collected.

k thank you sir,

if u dont mind can u provide any useful literature regarding to isolation of pesticides by hplc

which type of literature are u want.....

sir i mean how to analyse the samples by using hplc,