Using hexanol for microbial biomass fumigation instead of chloroform is not common, and there is limited research on its effectiveness for measuring carbon (C) and nitrogen (N). Chloroform is widely used because it efficiently disrupts microbial cells, releasing biomass components. Hexanol, being an alcohol, may not be as effective at breaking down cell walls, requiring adjustments to the method. Since there are few studies comparing hexanol to chloroform, conducting your own experiments to test its efficiency in extracting microbial C and N would be useful. You may need to optimize conditions like concentration, exposure time, and temperature for better results.