What are the most up-to-date methods for defining/mapping global land degradation and restoration?

I am seeking the best current methods and datasets (highest possible resolution) for defining and assessing global land degradation - ideally with a time series. I know there are different ways of...

24 November 2020 6,013 5 View

How do I write up thematic analysis results concisely?

Hello everyone, I've been writing up thematic analyses of interviews for the first time ever, and I can't help but write way too much for a thesis, let alone the papers that will make up a...

24 February 2020 5,044 11 View

Would anyone working in animal and wildlife research be open to answering a few questions I have regarding how you got to where you are in your field?

Hello, I am a current BSc student in Zoo Biology working towards a career in animal and wildlife research in the field. As apart of my course I am hoping to interview or get into contact with...

11 October 2018 3,710 3 View

How to compare two experimental data sets with only a few overlapping individuals?

I have two experiments from the last two years in the field. The experiments are supposed to be the same, but different environmental conditions slightly skewed the exposed conditions relative to...

16 October 2017 9,864 3 View

Generating cDNA from low yield RNA?

25 April 2017 1,040 3 View

Labelling monoamines with dansyl chloride?

Hi, I'm trying to observe where dopamine localises at the cell surface of my cells of interest but fluorescent dopamine is very expensive to buy. I've read online that I should be able to label...

12 October 2016 5,404 2 View

GFP expression in cell-free systems - Why dasherGFP can be expressed just fine in a cell-free system, while cometGFP doesn't seem to work?

Can anyone think of a potential reason why dasherGFP can be expressed just fine in a cell-free system, while cometGFP doesn't seem to work? I'm not sure if it could be a translation problem or a...

22 February 2016 2,231 4 View

Programs to identify specific Protein-DNA interactions?

Are there any programs out there that can identify which specific DNA motif a transcription factor binds to, given the entire promoter sequence and TF sequence/structure. I've got the promoter...

12 February 2016 2,630 3 View

What is the best way to isolate and grow bacteriophages?

I'm working with Cronobacter sakazakii and attempting to isolate it from baby milk and use phages to inhibit growth.

26 November 2013 9,168 2 View

Dear all, I was wondering if anyone knows whether MGLtools and Autodock can run on Mac OS?

I have tried to download the 64-bit version of MGLtools however, as many times as I have downloaded and uninstall the programs, an error arises that the app needs to be updated. Thank you.

03 March 2021 5,997 2 View

How to analyze a particular region of my simulation box in GROMACS?

Hi, I am using GROMACS 5.1.4 to simulate water in contact with a surface. I am only interested to calculate some properties of water near the surface. Let's say at a distance of x nm from the...

03 March 2021 9,989 2 View

I got 02 moderator values defining Johnson-Neyman significance regions. Is it an error?

Hi everyone, I am conducting research for my Master's thesis. I am using PROCESS by Johnson-Neyman to analyze my Moderator model. I test the relationship between Public Service Motivation and...

03 March 2021 2,350 2 View

How to get onecolumn document from standard IEEE ACCESS LateX template?

I have prepared a manuscript in IEEE Latex template, but for some purpose, I need to prepare a word file as well (detailed format is not required). Compared to two column .tex file, one column...

02 March 2021 1,830 3 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Do I need to strip the PVDF membrane before staining it with colloidal gold?

Dear Colleagues, After running Western blot on PVDF membrane and detection using ECL, I would like to stain my PVDF with colloidal gold to be able to allign the ECL image with total proteins on...

02 March 2021 7,829 3 View

How can the modification of garden soil cause global warming?

Hello, I am working on a soil project and I found a primary research article that tests how the modification of agricultural soil can cause global warming, using a thermal collector, however I...

02 March 2021 7,161 3 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

OpenBCI eeg--has anyone used them?

I'm looking to try out an off-the-shelf EEG set for some exploratory work. Curious if anyone has used the OpenBCI, and how much set-up work is required. I've heard they are more difficult to get...

01 March 2021 4,128 3 View