Hello! Please i need some help to troubleshoot my qPCR issues???

I have extracted good quality DNA (1339.3)ng/ul. I first run the normal PCR using same primer and i obtained very good result, but when qPCR was run , the result was totally disappointing. I...

04 May 2018 4,411 2 View

Hello ! Is it possible to use genomic DNA(gDNA) to check gene expression instead of using cDNA ?

Actually I m doing gene expression study and thought that we can save our time to directly use gDNA to check gene expression instead of following long protocols of RNA isolation and cDNA making

03 April 2018 5,746 6 View

Please What is the best dilution of cDNA from animal tissue for better qPCR result?

Relative quantification experiment Using samples From Liver tissue of Rabbits

11 December 2017 9,883 2 View

Please What is the best protocol for making standard curve from bacterial genomic DNA using Real time PCR?

08 September 2017 3,874 3 View

What degree of missing data in a particular case/participant results in Expectation-Maximisation no longer working?

I'm dealing with a mediation model and am using the PROCESS module in SPSS. Due to SPSS and PROCESS being limited in the imputation methods - being unable to handle multiple imputation - the other...

02 March 2021 4,362 1 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

Mixed ANOVA - help?

I'm currently working on a piece which asks to conduct an ANOVA - looking at the effect of sporting group (2 levels), and exercise trials (two trials with six levels). I've computed this all...

27 February 2021 9,626 4 View

How to Interpret Partial Eta Squared Numbers For a Repeated Measures Anova?

I have obtained Partial Eta Squared Numbers through Two-Way Mixed Anova Analysis. My results for different constructs are: .105, .135, .038, .068, .061 respectively. I wanted to know, whether...

24 February 2021 7,122 1 View

How to make a time series imputation benchmark ?

Hello ! In a work, I have to benchmark different algorithms to fill in missing values in time series. I insist on the fact that this is imputation and not forecasting. In my case, I have access...

24 February 2021 7,470 7 View

Interpretation of Goodman - Kruskal Gamma squared?

Dear colleages, Eta squared is dominantly used in reflecting the explaining power the same manner as a squared partial correlation coefficient (R2p ) from multiple linear regression: as the...

22 February 2021 7,801 3 View

Interpretation of a fluorescence Emission spectrum ?

Hello, i work with a spectrometer which only render the fluorescence emission spectrum. Until now i just found how to interpret an exitation- and emission spectrum together to generate deeper...

20 February 2021 1,291 3 View

In CDA analysis we have three stages, viz., description , interpretation, and reproduction. Why have all CDA studies neglected the third stage?

In CDA analysis we have three stages, viz., description , interpretation, and reproduction. Why have all CDA studies neglected the third stage?

18 February 2021 6,483 3 View

How can I interpret a ratio variable?

Dear colleagues, I am running a regression in which I have the following regression FoodExp = 67.04 DepRatio +.....e My interest is in the interpretation of the coefficient on the dependency...

16 February 2021 2,058 2 View

How do you interpret theoretical vs. experimental reaction orders with respect to a reactant?

I recently came across a paper focusing on the hydrodeoxygenation of oxygen-containing compounds. The paper linearized the rate equation in order to determine the experimental reaction order with...

14 February 2021 8,254 2 View