Ibrahim Bala Salisu

5 Questions 9 Answers 0 Followers

Questions related from Ibrahim Bala Salisu

Hello! Please i need some help to troubleshoot my qPCR issues???

I have extracted good quality DNA (1339.3)ng/ul. I first run the normal PCR using same primer and i obtained very good result, but when qPCR was run , the result was totally disappointing. I...

05 May 2018 4,444 2 View

Hello! Please, how does fold induction differ from fold change? and which of the terms is best used for qPCR result interpretation?

I`m working on gene expression study and sometimes the two terms confused me.

05 May 2018 2,992 1 View

Hello ! Is it possible to use genomic DNA(gDNA) to check gene expression instead of using cDNA ?

Actually I m doing gene expression study and thought that we can save our time to directly use gDNA to check gene expression instead of following long protocols of RNA isolation and cDNA making

04 April 2018 5,784 6 View

Please What is the best dilution of cDNA from animal tissue for better qPCR result?

Relative quantification experiment Using samples From Liver tissue of Rabbits

12 December 2017 9,916 2 View

Please What is the best protocol for making standard curve from bacterial genomic DNA using Real time PCR?

I want to compare the Traditional CFU count of bacteria with Real-time PCR method, but I m having some issues with the making standard curve. So please if anyone is willing to help I will really...

09 September 2017 3,925 3 View