23 January 2024 0 7K Report

It will be different from the fish's respiratory frequency (Number of closed-open of fish's gill per unit of time)

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Does it work as antibiotic resistance if plamid has no RBS??

I am on gene work to express gene of interest in some Bacillus species. Thus far, i need to try some RBS in Vector which is optimized for bacillus subtilis. Before i design vector to express in...

11 July 2024 7,847 1 View

Any low-cost mid-infrared spectrometer?

If we build up a mid-infrared laser system and want to measure its spectra, where can I find the lowest-cost mid-infrared spectrometer, ranging from 2 to 20 μm。 Any recommendation? Thanks!

26 May 2024 8,114 1 View

What is the meaning of intersection point in transfer curve ?

Hi, I'm doing research n-type neuromorphic transistor device. According to theory and other research, forming EDL (electro double layer) can cause anti-clockwise transfer curve. However, as you...

26 May 2024 5,402 1 View

How to calculate land use based on the plant capacity?

I want to estimate the land use of a fermentation plant, and how to get the covered area based on the plant capacity roughly, is there any available models to make a rough calculation?

20 May 2024 8,589 0 View

Can I insert RBS and His tag in primer to clone interested gene????

I designed primer to clone the target gene with RBS, his tag. I want to confirm below: 1. if Is it okay that extra sequence longer than target gene part?? 2. I designed as RBS located after his...

10 May 2024 7,557 2 View

Can I insert RBS and His tag in primer to clone interested gene????

I designed primer to clone the target gene with RBS, his tag. I want to confirm below: 1. if Is it okay that extra sequence longer than target gene part?? Targeted gene part is 18bp long But...

10 May 2024 3,106 4 View

Hello, i need help about the name of this lobster, can everyone help me?

Because this lobster by at local market so it dont have enough claws and legs, i hope everyone can help me the name! thank you very much

16 March 2024 9,624 0 View

How to know if RNA extracted from the desired brain area?

Hi! I am trying to do RNA extraction from p7 mice's somatosensory cortex and hippocampus. But I am not sure if the RNA was extracted from the correct brain region. I was wondering if there are any...

25 February 2024 5,484 1 View

Why my gel bands are happening like that?

When I run the gel for the first time, everything went well. But this time, I don’t know what’s wrong with my gel result although I use the same procedure and method. The band should be ~600 bp...

18 February 2024 1,446 0 View

Kruskal-wallis post hoc test, Dunn's or Mann-Whitney U with Bonferroni, or DSCF? Opposite results?

Dear all, I encountered a weird situation in my research. I have 3 groups, they are all non-parametric and no equal variance as well. I ran a Kruskal-wallis test to compare the difference and my...

06 February 2024 9,454 4 View

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Aspen Plus Simulation?

Greetings When one is to perform Simulation or modelling of Pyrolysis of Biomass Waste materials using Aspen Plus software, and one has both the macromolecules breakdown (Cellulose, Lignin and...

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How to quantify polystyrene microplastic (8 micron) bioaccumulation in fish tissue?

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Why results of ROS flurescence are negative as there was no bacteria within?

Hello. I am working on ROS production of two systems: system A is cerium oxide and hydrogen peroxide, system B is cerium oxide nanoparticle, hydrogen peroxide and potassium bromide. I did some...

04 August 2024 5,974 3 View

Why aren't most metal oxides insoluble in water?

Why BaO (s) + H2O(l) ---> Ba 2+(aq) + 2 OH - (aq) if Oxygen loves owning all the electrons, why would it bind to hydrogen which competes with Oxygen for electron ownership more so than...

03 August 2024 5,392 1 View

How to Transform my handy-wavy explanation of why group I/II metals oxides in aqueous solution are basic into a rigorous argument​?

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What is the cycling of matter through ecosystems and how energy drives the cycling of matter in photosynthesis and cellular respiration?

What is the cycling of matter through ecosystems and how energy drives the cycling of matter in photosynthesis and cellular respiration?

02 August 2024 9,518 2 View

¿Are oxygen vacancies in semiconductors capable to change the band gap?

Dear all! Oxygen vacancies do raise negatively the Fermi level by increasing the negative charge in the semiconductor crystal structure. In the case of n-type semiconductors, I wonder if this...

30 July 2024 8,339 2 View

Do anyone knows any book dealing with fish growth/zootechnical performances and somatic indexes in aquaculture?

In the aquaculture and fish nutrition research field, a number of growth and somatic indexes are often used, including specific growth rate (SGR), feed conversion rate (FCR), protein efficiency...

22 July 2024 2,480 4 View

Why do fish have fishy odors naturally ?

Chemical Engineering /Food Engineering

20 July 2024 4,152 4 View

Chromosomal spreads are shrunken after fluorescence in situ hybridization (FISH), why?

Dear colleagues, We are trying to do FISH on mice PDEC metaphase spreads. We use homemade biotin labelled probes, synthetized using a nick translation kit. For hybridization, initially we were...

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