How to mutate a DNA sequence to stop binding to its specific primer?

I am doing reverse transcription using a semi-suppressive PCR therefore I introduce a template switching oligo at the 5' end of my transcript which is complementary to the PCR handle at the 3'...

03 October 2018 4,282 2 View

Is anyone aware of a plugin out there to perform discriminant analysis in PSPP?

If so, could you point me in the right direction? Can't seem to find anything.

09 January 2014 3,600 1 View

Need help making a Fiji (ImageJ) Tool

I'm working on manually analyzing a series of photographs of archaeological artifacts. Each photo contains a series of stone tool fragments laid out on a neutral background. I'm using Fiji image...

06 January 2014 5,335 10 View

Any common protocol for inflammatory stimulation of murine endothelial cells in vitro?

26 December 2013 1,701 9 View

Can the measurement of cytokine levels according to their half lives be useful?

A question of cytokine kinetics. In an activation assay, we usually want to measure effects of our treatment (whatever this might be) on pro- or anti-inflammatory cytokines. Most commonly...

28 November 2013 2,096 3 View

MPO activity assay - can anyone help?

I homogenized murine lungs in 0.5% w/v HDAB in 50mM sodium phosphate buffer to perform a MPO activity assay. The protocol I do have instructs that I should use 20ul of my samples with 160ul TMB...

01 October 2013 8,409 3 View

How fast should an ELISA plate be read?

I finished an ELISA assay and read it to discover two hours later that I saved the 630nm data which I used to correct my 450nm data. I immediately measured the plate again but I'm not sure whether...

05 September 2013 4,109 21 View

qPCR data representation

I have some qPCR data which I want to represent in graphs. I used to use the DCt values but the problem is that they are reciprocal to the expression changes (high DCt values means low expression)...

13 July 2013 4,875 2 View

What are your experiences with conducting (and publishing) online surveys?

I am interested in conducting an online survey and curious as to the requisite ethical approval and the receptiveness of peer-reviewed journals to the results. If anyone has any experience in...

02 July 2013 8,531 40 View

Which transcription factors are activated early in response to inflammatory stimuli?

04 June 2013 6,343 34 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Does the pressure(-600 mmHg) affects pH?

When I conducted the degassing experiment using decompression under anaerobic condition(and I used ferrous based chelating agent), the oxidized ferric were observerd. So, could you tell me the...

03 March 2021 4,892 5 View

What are the most reliable approaches to extract key continuous frames from a video, with complete automation?

I have an input video, of skin (from a microscope that means it is magnified such that blood capillaries are visible). The idea is to extract 15 sec of continuous frames from a video of 1 min,...

02 March 2021 2,735 2 View

Which is the best place in the world for the plant biotechnology research?

I am searching for a good place for the Post Doc,

02 March 2021 4,053 3 View

How can extract ciprofloxacin hydrochloride disolve in water er to organic solvent such as dichloromethan or any other solvent?

I need a procedure to extract the ciprofloxacin hydrochloride from aqueous solution by using dichloromethane in detail,. I thank you very much.

01 March 2021 4,306 1 View

Which bacteria are suitable for quorum sensing screening?

I want to find a quorum sensing inhibitor from plant extracts. Klebsiella pneumoniae, Moraxella catarrhalis, Staphylococcus aureus, and Streptococcus pneumoniae. I looked for these strains...

01 March 2021 7,675 3 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

Isolates of Hymenoscyphus fraxineus?

Dear Dr. Cai, my name is Simone Prospero and I work in the team of Phytopathology at the Swiss Federal Institute for Forest Snow and Landscape Research (WSL;...

01 March 2021 1,133 1 View

How to avoid ulcers in s.c. injected tumors ?

Recently, we have injected s.c. B16F10 tumor cells in BL/6 mice. At around 12-15th day some animals showed ulcerations in tumors , with a big hole in the middle (it looks like a volcano). We have...

01 March 2021 9,938 3 View

How to perform statistical analysis of coefficients extracted from transform techniques?

I am studying the potential of DCT and FFT coefficients extracted from a signal to classify the different objects that emit the signal. I applied the Shapiro-Wilk to confirm that data was not...

01 March 2021 8,079 2 View