Please suggest me which universal primers should I use to identify fungi upto species level?

10 November 2016 8,952 4 View

Can we amplify gene of interest from cDNA or mRNA using single degenerate primer?

I want to clone my gene of interest from a eukaryote.....I have a single 5' degenerate primer available to me. can I use single 5' degenerate primer from my amplification and oligo T as my reverse...

01 February 2016 5,835 4 View

Do we have to add extra base in front of ATG of the desired protein when cloning in BamHI site of pET22b?

I have cloned a PCR product into BamHI, Not1 site of pET22b but I am not seeing the protein. Do I have to add an extra base in front of ATG of my gene in order to make it in frame or can I use...

11 December 2014 5,425 2 View

Can anyone suggest as to why I am not able to see the expression of 45KDa protein in pET22b?

I have cloned my genes of interest in pET22b and still not able to see the expression in BL21(De3)plys.

10 November 2014 7,866 8 View

Can anyone help with phosphate estimation problems?

I am getting the dark blue colour even before adding phosphate to my reagents when estimating phosphate with ascorbate method. Could someone troubleshoot this for me?

04 May 2014 3,274 5 View

Does anyone have any suggestions for cloning the entire genome of an RNA virus?

I want to clone the whole viral genome from a water sample from which I have concentrated the virus and done DNA and RNA isolation. I want to find the diversity of viruses and therefore would like...

04 May 2014 6,611 2 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What are some of the research gaps in machine learning and artificial intelligence in africa?

i would to know some of the research gaps in the artificial intelligence field in most african countries.

03 March 2021 6,145 3 View

How to access MSCI KLD Stats and Sustainalytics?

Hello all, I'm currently working on my undergraduate thesis and I'm having difficulties trying to access KLD Stats and Sustainalytics because my campus Airlangga University and apparently other...

02 March 2021 8,525 1 View

Ideas for Phd's Thesis on pricing (Marketing)?

Hi everyone, I'm studying Marketing and I would like to write my PhD thesis on the topic of pricing. Any specific ideas?

02 March 2021 9,706 5 View

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02 March 2021 3,060 3 View

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02 March 2021 932 1 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

How to calculate BREQ 3 questionnaire?

how to calculate BEHAVIOURAL REGULATION IN EXERCISE QUESTIONNAIRE BREQ 3 ,which contain 24 questions and each question has 5 answers (0- Strongly disagree , 1-disagree, 2- Neither agree nor...

02 March 2021 811 3 View

Please suggest a good text book for "Research Methodology in Life Sciences". Which book would be good for PhD Scholars?

Please provide Book Title and author name

02 March 2021 9,059 3 View

How does Scientist/RG community evaluates a case study article and the impact of it on a researcher career?

An article that is published as a case study in the SJR listed journal. How much value is added to a researcher career? How it is evaluated by a scientific researcher/University admission...

02 March 2021 9,661 3 View