Vikas Sharma

7 Questions 12 Answers 0 Followers

Questions related from Vikas Sharma

Please suggest me which universal primers should I use to identify fungi upto species level?

I have isolated 10 different fungi from rhizosphere and would like to identify them upto species level.

11 November 2016 9,038 4 View

Can we amplify gene of interest from cDNA or mRNA using single degenerate primer?

I want to clone my gene of interest from a eukaryote.....I have a single 5' degenerate primer available to me. can I use single 5' degenerate primer from my amplification and oligo T as my reverse...

02 February 2016 5,889 4 View

Do we have to add extra base in front of ATG of the desired protein when cloning in BamHI site of pET22b?

I have cloned a PCR product into BamHI, Not1 site of pET22b but I am not seeing the protein. Do I have to add an extra base in front of ATG of my gene in order to make it in frame or can I use...

12 December 2014 5,479 2 View

Can anyone suggest as to why I am not able to see the expression of 45KDa protein in pET22b?

I have cloned my genes of interest in pET22b and still not able to see the expression in BL21(De3)plys.

11 November 2014 7,928 8 View

Hello everyone....

I am looking for a post doctoral position in Microbiology and currently i am pursuing post doctoral fellow in Department of Microbiology University of KwaZulu Natal (Westville campus) Durban South...

07 July 2014 1,856 1 View

Does anyone have any suggestions for cloning the entire genome of an RNA virus?

I want to clone the whole viral genome from a water sample from which I have concentrated the virus and done DNA and RNA isolation. I want to find the diversity of viruses and therefore would like...

05 May 2014 6,668 2 View

Can anyone help with phosphate estimation problems?

I am getting the dark blue colour even before adding phosphate to my reagents when estimating phosphate with ascorbate method. Could someone troubleshoot this for me?

05 May 2014 3,338 5 View