I want have a better idea of the principal approaches that can be analyzed using mitochondrial DNA sequences in vertebrate evolutionary study.
In particular I am interested in "the taxonomic distinctness measure". Is there any software where this analysis can be done? Best regarts Cristian
05 June 2017 8,535 2 View
I would like to know which software is the most commonly used or recommended for this analysis. Best regards, Cristian
02 March 2017 5,247 3 View
Greetings to all.Does anyone know the size (base pairs) of the following primers of the mitochondrial Cyt b gene? MVZ11/MVZ28MVZ05/MVZ08MVZ21/MVZ16 Best regards and thanks Cristian
11 December 2016 7,962 0 View
Hello everyone, I want to make an analysis using DNA mitochondrial sequences to further examine the history in some mammals populations in Mexico. Thanks and best regards, Cristian
08 September 2015 5,222 0 View
I am currently working with mitochondrial DNA sequences. Thank you very much for your help and time. Regards.
31 December 2014 7,190 2 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...
08 August 2024 4,645 2 View
After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
08 August 2024 1,668 3 View
I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you
08 August 2024 4,733 2 View
Vertebrates and invertebrates (e.g., arthropods: insects, crustaceans, etc.) have two different neuro-muscular systems given their differential construction: the former have an endoskeleton and...
07 August 2024 648 0 View
I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...
05 August 2024 9,059 3 View
I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...
01 August 2024 2,079 0 View
I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...
30 July 2024 6,420 1 View
Hi all. As a beginner in ChIP-seq experiments, I hope you understand that the following questions might be somewhat basic. I am planning to perform ChIP-seq or MeDIP-seq analysis to investigate...
28 July 2024 6,938 1 View
It has been documented that apoptotic cells themselves can induce phosphorylation of serine 139 on H2AX (γH2AX) due to DNA fragmentation during apoptosis (doi: 10.1074/jbc.275.13.9390). As γH2AX...
28 July 2024 7,983 2 View