In Africa many young graduates are not confident for their future due to unemployment after graduation. can you please advice them?

having reasons leading to solutions of this problem

06 July 2018 7,164 1 View

Is there any importance of Marriage out of having children?

having knowledge on daily discussion of the topic

05 June 2018 4,920 2 View

What is the origin of wisdom?

i am writing a paper

05 June 2018 7,942 11 View

Can anyone share with me the new version of APA referencing style?

want to share it with colleagues who teach research in institution. Thanks

05 June 2018 5,200 14 View

Do you wish to contribute on our confrere?

we have a conference on non communicable diseases at INES-RUHENGERI, Rwanda on 28th and 29th of August 2018. ideas, collaborations and funding opportunities, welcome! Callixte

05 June 2018 2,576 0 View

How do you think on immunity of babies born through normal delivery and C-section?

i am doing research on this topic

03 April 2018 2,394 2 View

Hello Dear Researchers and academicians, can anyone tell me how some bacteria increase risks of obesity during pregnancy?

Research

03 April 2018 2,800 2 View

What do you think on state of someone after death?

i am leading the mind on philosophy of life

10 November 2017 7,176 0 View

Hello Dear researchers, do have you ever thought on factors leading to sickle cell diseases out of genetic transmission?

i am working on this research in Uganda and East Africa

09 October 2017 7,580 0 View

Hello Dear Colleagues, can any one help me on existing data related to food contamination mortality prevalence?

07 August 2017 7,420 0 View

What is the exact mechanism of the antimicrobial effect of local anesthetics?

The antimicrobial effect of local anesthetics has been known for more than 50 years, but it still seems to have an unclear mechanism of action. which bacteria? which concentration? etc etc. ....

27 February 2021 4,450 2 View

Uncertianiny in using dry or wet basis in mixing a sediment with fuel oil?

Hello I'm currently working on a topic related to studying the anaerobic biodegradation of TPH in marine sediments. In this work, sediment should be mixed with fuel oil to a concentration of 20 g...

24 February 2021 9,839 3 View

Advice on growing lactobacillus species in the lab?

Hello everyone, For part of my PhD project, I'm hoping to grow some lactobacillus species in the lab so that I can create standard curves for a qPCR quantification of a faecal sample. However, I...

24 February 2021 3,157 6 View

What are the best cations for preparing competent cells, Ca2+ or Mg2+?

I have been reading various articles and most state that treatment with CaCl2 produces higher efficiency of transformation than MgCl2. Can anyone explain why Ca2+ is better than Mg2+ at...

16 February 2021 8,519 5 View

Would using an asRNA for repressing transcription of a high expression Listeria monocytogenes non-coding RNA harbored in a plasmid be a viable option?

Hello all, I am interested in deleting a highly expressed non-coding RNA located on a Listeria monocytogenes plasmid. Currently, there are not any optimized gene deletion methods for Listeria...

09 February 2021 4,994 1 View

Review Papers regarding transcriptional regulations & promoter types/classes in Gram positive bacteria?

Dear fellow researchers, Could you please suggest me excellent review papers discussing transcriptional regulations and/or promoter classes in Gram positive bacteria? Thank you so much for your help!

08 February 2021 6,909 3 View

Flexibility of RNAseq?

When you use RNAseq I understand that it must follow certain conditions when you use it. One of them is the insertion of a polyA tail. The insertion of the polyA must include then...

05 February 2021 3,770 4 View

How do microbes tell the presence of an inorganic minaral in the soil?

Due to their ubiquitous nature, there is a tendency of the presence of microbes in a given mineral(s). For instance, if microorganisms that love the of pH less than 6 in the pH value, is isolated...

03 February 2021 4,630 10 View

Moving bacteria-like organisms in my HEK293T cell culture?

Hi everyone, I have been culturing HEK293T cells that have been engineered to overexpress the mechanosensitive protein Piezo1. However, I am finding it very difficult to keep them in culture -...

02 February 2021 7,892 4 View

Does anyone have a copy of the DIBaS dataset?

I am a student from Brunel University London, and I was looking to use the DIBaS dataset for my dissertation. However, recently it has not been accessible via this link:...

26 January 2021 5,185 4 View