I am working on my thesis work, hepatic biotransformation of some biactive compounds extracted from natural products in rat microsomal fraction (S9), for which I need to know the possible enzymatic reactions that will happen and the probable structures of the metabolites.
The body produces a steady stream of metabolic waste products (ammonia, bilirubin, urea, lactic acid, etc.)
along with toxic byproducts called – exotoxins - as a result of microbial activity in the human intestine. Continual exposure to toxins originating from both within the body and outside the body gives credence to one of the body’s most vital functions – hepatic biotransformation. The sole purpose of the biotransformation process is to convert toxic compounds into non-toxic, water-soluble compounds which can easily be eliminated. The liver (hepatic) is the key player
in this process.
Detoxification - for many years the term “detox” referred to breaking free of alcohol or drug addiction. Nowadays,
detox means removing all toxins from the body; not just poisons from substance abuse, but also heavy metals, chemical additives, and other toxins found in our food, water and air. As well as the metabolic waste products produced by the body.
Biotransformation - is defined as a metabolic process whereby chemical modifications or alterations are made by the body of a specific chemical compound, usually by means of enzymatic activity. As mentioned these chemical compounds originate either from within the body, usually the result of metabolic waste and are called endogenous compounds or they come from outside the body and are called exogenous compounds.
There are different techniques for structural elucidation of drugs provided that you have pure component. High resolution MS/MS, NMR, X-ray.
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