Hello! Are there any reference intervals for serum P1NP and CTX1 among Indian adolescents?

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Can I use two different ranges of 5-point Likert scale (0-4 and 1-5; adapted from two different authors) in my questionnaire?

Good day everyone! I have searched and read a lot of topics about this one and yet there are still varying answers left and right. I need a specific one so I hope someone can help me with this. I...

02 July 2024 1,772 2 View

Background on the project topic gender and sports participation in cape coast metropolitan?

an introduction

02 June 2024 520 0 View

How do I correlate two sets of 5 point Likert scale questions?

Hello everyone. I have 2 sets of questionnaires with the following scale choice: (1) Poor - Excellent (2) Seldom - Always Both are 5-point Likert scale. The first scale has 10 items, asking for...

22 May 2024 9,845 5 View

Thesis on the effect of stress on employees performance and productivity in Ghana?

I will be grateful if i can get the latest work on the above topic.

17 May 2024 2,509 1 View

What is the research gap about the title topic?

title topic " Role of Facility Layout in enhancing workplace safety and ergonomics in Batangas State University"

06 May 2024 8,388 1 View

Please help. What is the name of the lock acquired before entering the soft interrupt in the Linux source code?

My experimental environment is Linux6.1, and the real-time patch preempt_rt has been applied to improve the real-time performance of the network. I checked the relevant papers and saw a lock...

18 April 2024 8,721 3 View

What is the relationship between anticoagulants used in blood collection tubes and lactate dehydrogenase (LDH) activity in plasma or serum samples?

This question is derived from an article entitled "Underfilling of vacuum blood collection tubes leads to increased lactate dehydrogenase activity in serum and heparin plasma samples" by Neuwinger...

08 April 2024 8,391 1 View

Does Sysmex DI-60 facilitate remote access for users in different geographical locations? Does it have features for troubleshooting?

I am interested in the functionality of the Sysmex DI-60 system in terms of its remote access capabilities and troubleshooting features. Particularly, I am curious to know whether this automated...

01 April 2024 5,516 1 View

Different species, same matrix, can I make different way to make STD and QC samples during method development and validation?

There days I was facing one challenge when doing MD and MV. I need to use rat and pig' plasma as matrix. I used to spike the solvent with different concentrations into the plasma to make STD and...

01 April 2024 3,766 0 View

How much DNase I is too much?

I am using the Thermo Scientific DNase I (https://www.thermofisher.com/order/catalog/product/EN0521) to remove genomic DNA from RNA extracts eluted in nuclease free water. The protocol calls for...

30 March 2024 7,100 0 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

CHO-K1 suspension adaptation protocol?

I am trying to adapt adherent CHO-K1 cells in F12K+10%FBS to suspension culture in only F12K, but in vain. I have followed Mirus bio as well as Thermo protocols, please suggest any alternative....

31 July 2024 3,756 0 View

How to prevent hemolysis after performing cardiac puncture to draw blood from mice in serum?

We have encountered an issue with the serum of mice being hemolyzed after the process of centrifugation. Could you please provide guidance to prevent hemolysis ?

29 July 2024 8,402 4 View

ELISA sandwich with two monoclonal antibodies ?

hi, I am facing difficulties in the développement of an ELISA sandwich. I have several experiences in developing ELISA but that’s the first time I can’t find any solution. My test is currently as...

16 July 2024 9,044 10 View

FACS markers to characterize rat bone marrow-derived macrophages?

Dear all, I am looking for FACS markers to characterize my rat bone marrow-derived macrophages (M0). They were differentiated from monocytes with M-CSF. I am considering to use CD11b/c and...

15 July 2024 4,615 1 View

Why is the baseline fibrosis level of HK-2 cells very high?

In many experiments where HK-2 cells were stimulated with TGF-β at 10 ng/mL for 48 hours, I observed that even in the control group, the baseline expression level of fibrosis proteins like FN1 and...

15 July 2024 1,929 2 View

Unexpected 35 kDa Band Observed During Expression and Purification of Dengue 3 NS5 RdRp Domain ?

Background: I am developing an RdRp assay for Dengue virus-3 and need to express and isolate the NS5 protein's RdRp domain (~101 kDa). My expression vector includes a GST tag followed by a 6x His...

13 July 2024 9,950 3 View

The HCP removal rate is lower than usual in concentration step, why?

Hello everyone. I am working on my LV concentration porject, I use 500Kda hollowfiber to do it. The HCP and BSA removal rate is nearly 80%, but last time, I failed to run it. The HCP hasn't been...

11 July 2024 5,380 0 View

Comparison of tissue to serum MS data?

Hello, So I have mouse brain tissue MS data from one of my predecessors and I have human serum data from the hospital that I collaborate with. We are trying to find markers for the diagnosis of...

11 July 2024 8,484 0 View

I seed my cell in Opti-MEM?

I seed my cell (KG1a )in opti-mem media for transfection (without lipofectamine as control ) and after 2 hours I add FBS 10 ul in each wells all my cells after 24h in control wells or with...

07 July 2024 407 0 View