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05 June 2016 1,543 17 View

How philosophy can explain : "Love is so strong as the death" (Song of songs 8,6)?

02 March 2016 7,653 3 View

How can you explain or make clear that the narrative story is a way to make to emerge the ethical conscience?

I research works in narrative story and it's use in palliative care, and I try to make any relation with Ricoeur's worth narrative identity

10 November 2015 2,558 0 View

In Ethic how is the relation between the concept of responsibility and the time expressed?

07 August 2015 5,285 4 View

When does Dignity of human being begin and end? What does this notion implies (human rights)? Is dignity specific to human?

09 October 2014 4,951 3 View

Which links exist between the logic in De Interpretatione and the ethic of Nicomac, between truth and good for Aristotle?

09 October 2014 6,221 30 View

The "pure" I in Husserl's phenomenology has a real purpose?

09 October 2014 3,657 1 View

How to determine implicit ontological and epistemological stance?

I am hoping for some guidance and additional reading material that will assist me in determining ontological and epistemological stance when not explicitly detailed within the research. Here is...

26 February 2021 227 5 View

Trouble shooting with his tagged protein expression in E.Coli?

i have issues in expressing a chicken protein in BL21 cells with his tag. I do not see induction even at 18 degrees. Any suggestions

15 February 2021 8,939 3 View

During an anti-His capture ELISA has anyone had issues with histidine residues from secondary antibodies non-specifically binding?

Hi, Hope you can help. Currently been running some sandwich ELISA assays where I coat with anti-His, block and then capture His-tag protein and then sera and then anti-bovine monoclonal...

07 February 2021 7,101 1 View

How to clean up an already pure protein from grwoth medium?

Hi all, I have got a protein, expressed in P. pastoris vector, more than 97% (SDS-page) pure in the culture medium after cells separation. Despite the protein expressed has a his-tag, Nichel...

04 February 2021 5,173 6 View

How can a traditional medicine exist if its validated outcome is translated into molecular mechanism for acceptance of modern medicine?

A traditional medicine (e.g. Unani System of Medicine) has its own philosophy of treatment of a disease. Nowadays, there is emerging demand to validate the effectiveness of an intervention (a...

05 January 2021 9,472 5 View

You can mention a contribution that science will achieve in this year 2021?

It is known that scientific knowledge is replicated each time at more unusual speeds, however, humanity expects more from our scientific work, that is, solutions to problems that have accumulated...

02 January 2021 8,300 16 View

Is there any cheaper IoT workload/stress testing tools?

Previously, I decided to use iotify.io as the testing platform for IoT application. They provide MQTT/HTTP, etc and well documented & visualization, but it spent too much cost for me because I...

28 December 2020 5,911 3 View

What is your philosophy about life ?

how do you understand life as it is really big suspense, why we are here, our consciousness is big unconsciousness and our unconsciousness big consciousness.

25 December 2020 7,632 19 View

What the essence of yoga and meditation ,how you take it as?

we all have our own thought and experience regarding yoga and meditation, we all know it's a miracle unexplainable to those who do he/she feel it.What's your view regarding it.

24 December 2020 3,615 10 View

Why the taste of foods(item) changes with the sources of fuel we used(bio fuel,wood,dried dung) reality or meth ?

As my experience and many consumer experience they feel the different taste of the same item served cooked in a different source, is it real or not, what is the reason behind this.

24 December 2020 6,476 3 View