Will a 10xHis-tag prevent anti-MBP from binding to MBP?

I have a protein with a 10xHis-MBP tag on the N-terminus. I'm fairly certain I am expressing my protein, but I'm not getting a signal on my western blot. Is there a possibility that the 10xHis tag...

25 February 2021 8,404 3 View

Cyclooctatraene damaged by TFA?

16 February 2021 4,747 3 View

Inexpensive eye tracking hardware + software solution?

Hello, I am starting a project that will require an eye tracking device. I've seen eye tracking hardware + software solutions that are $10,000+ so I am hoping to find one that is significantly...

18 January 2021 8,121 12 View

What materials besides gold can be infused into window panes?

Hello, Driving around, I have noticed a lot of office buildings where the glass panes have been infused with gold. The thin layer of gold reflects the sun and keeps the office at a comfortable,...

06 January 2021 976 4 View

Why build a Leaning Tower of Lire?

What are the lessons to be learned from building a leaning tower of Lire?

03 January 2021 7,364 4 View

What are some of the applications of transparent wood?

Are there any particular uses for transparent wood other than visual appeal? The transparent wood is really cool to see, but what are the practical uses? Please provide examples. Happy New Year!

31 December 2020 2,479 4 View

Can light waves induce electricity through induction?

Can light waves induce electricity? I am unclear on this matter. Thank you.

30 December 2020 7,974 3 View

What are the benefits of applying Blockchain within any industry?

Is blockchain only for cryptocurrency? Thanks in advance.

28 November 2020 5,086 11 View

Is my Chi-square test valid?

18 November 2020 3,033 2 View

Confused primer dimer or something else?

Hi everyone, i recently did a qPCR to check the best annealing temperature for my primers. So i ran two of my primers on annealing temperature 59C with a melting curve. The melting curve shows...

17 November 2020 1,913 5 View

What is the difference between L1 and L2 regularization in machine learning?

L1 and L2 regularization

28 February 2021 4,187 3 View

How to immerse bricks in wax ( for coating ) ?

Do we immerse the bricks horizontally or vertically ? #bricks#waterproofingsurface#civil#engeenering#chemicalengineering#wax#parafinwax

28 February 2021 6,685 2 View

How to analyse a (perceptual) study using differing/comparable sets of stimuli?

I plan on running an online perceptual experiment that has, let's say, 100 unique video stimuli altogether. Since these are too many to be rated by every participants, I would like to present the...

15 February 2021 7,444 2 View

Why is PVDF splashing and bursting under vacuum ?

I prepared PVDF in a very regular way by dissolving it with stirring at room temperature but when i placed it in vacuum for eliminating the bubbles and trapped gases it started to burst and...

09 January 2021 3,965 3 View

Is concrete block bricks eco-friendly?

Concrete block bricks companies are marketing that their products are eco-friendly, as they are not emitting carbon dioxide and not using soil. At that viewpoint, they are right. But,in their...

04 January 2021 5,641 8 View

How would you scale intra-day returns to daily returns?

16 December 2020 6,284 3 View

HowScholarships, Fellowships and grants in Materials engineering for international students to study abroad?

On Line student doctorant looking for first Master PhD student doctorant as UNICAF now in Materials engineering for international students to study abroad on Line and To Bricks LTGS and To have...

10 December 2020 3,686 3 View

A Hybrid accountant and the Fourth Industrial Revolution(4IR), redefining the business advisor and value addition?

Can anyone provide viable research questions on the following; Formulate the PhD Questions from the elements below: Effect of the 4th industrial revolution - On the Accounting profession - And...

03 December 2020 5,533 4 View

What is the good water absorption value for construction materials (brick, mortar etc.)?

According to many construction standards such as ASTM, a good construction material should have a water absorption value within 20%. However, if we compare with many water absorption values (from...

26 November 2020 8,538 8 View

What do you think is the difference between distance learning and regular education?

Which do you think is more effective, distance-learning, or regular training?

23 November 2020 7,162 21 View