Have you reviewed the evidence for using polymeric membrane dressings (originally designed for pediatric burn patients, continuously cleans, etc.)?

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Experiment or Quasi Experiment Design?

What kind of study design are we talking about? Subjects are randomly assigned to one of three groups. In addition, a moderator and a mediator are collected as continuous variables. Is this an...

15 January 2021 1,809 4 View

What is the best solvent for the extraction of chemical compounds that have an insecticidal effect ?

26 November 2020 4,609 15 View

Nvivo - how to extract and analyze hashtag?

Hello, I am using Nivo 12.0 plus. I've a dataset of tweets, unfortunately not extracted by NCapture. How can I extract and analyse hashtag? Is it possibile using auto-code functions? Thank you

19 August 2020 9,532 3 View

What is a scoping review?

In what context should we choose to do a scoping review? How is it different from systematic review, literature review, mapping review and Cochrane review? What are its mandatory or optional...

05 July 2020 8,206 3 View

If I use REVEL score as a meta-predictor for clinical variant classification, what would be a reasonable cut-off to filter disease variants?

Ioannidis et al., 2019, reported the development of the REVEL score (rare exome variant ensemble learner) for redefining pathogenic variant classification, and Tian et al., 2019, reported that...

29 March 2020 2,450 2 View

Is there a method to statistically compare two or more regression coefficients in paired data?

Hello, I have conducted simple linear regression for four time points, always on the same sample. My time points are 10 years apart and now I wish to compare the slopes / betas / regression...

21 March 2020 10,020 9 View

Is the Case Study Method eligible to create theory?

I have been conducting research for PhD Theses using case study as a method, mainly refer to Robert K. Yin works. At the final step of my research, I define some concepts and use the concepts to...

12 February 2020 3,146 10 View

What is the blue-shift in UV-vis?

hello, Im having trouble in extracting information from my uv-vis data. The sample the I analyzed is protein corona. Before that, I already characterize 40 nm of gold nanoparticle and obtain its...

15 May 2018 8,815 7 View

Does someone have neuronal cell cultures of zebrafish?

I plan to use immunohistochemical methods to stain brain sections from adult fish (teleosts, starting with zebrafish). In order to validate the antibodies I would like to test them in a 2D-celll...

10 September 2017 6,280 1 View

Please does anyone knows how to use genome mining in search of genes encoding for particular enzymes?

For instance if I want to search for genes encoding particular enzymes which bioinformatic tool is better and how do I select the best genes to investigate?

14 August 2017 1,967 4 View

POlymer+DMSO solution and moisture??

What could be the reason of moisture at the sides of Vial when I am preparing my polmyer+DMSO solution for membrane fabrication? What etiquettes I am possibly missing here?

28 February 2021 6,516 5 View

IF troubleshooting: looking at an intracellular protein that localizes to the cell's and how to reduce increase in signal due to cell ruffles?

I am currently trying to stain for PI3KBeta in MDA-MB-231 cells. My current issue is that 1) beta stains all over the cell and 2) there is an increase in signal at the edge of the cell and this...

24 February 2021 8,582 1 View

Cloudy/high background western blot - can anyone help me?

Hi everyone! I try to detect VDR protein (~48 kDa) using Western Blot. After SDS-PAGE using 10% MP TGX Stain-Free Gel from BioRad I transferred the protein onto an metOH activated PVDF membrane...

23 February 2021 6,266 3 View

What are the best cations for preparing competent cells, Ca2+ or Mg2+?

I have been reading various articles and most state that treatment with CaCl2 produces higher efficiency of transformation than MgCl2. Can anyone explain why Ca2+ is better than Mg2+ at...

16 February 2021 8,519 5 View

I have some problems with the mass transfer part of the CFD simulation of membrane distillation.Can anyone help me?

I am working on modeling of Direct Contact Membrane Distillation system with COMSOL Multiphysics software. I have some problems with the mass transfer part of the simulation because I don't know...

14 February 2021 379 2 View

Regarding immunology, transmissivity, herd immunity and vaccines in Sars CoV-2?

This is a follow-up on an earlier question regarding mechanistics of vaccines: why current vaccines don't block transmission. In summary, they don't engage mucosal immune cells to produce IgA....

08 February 2021 8,321 3 View

Background and spots in Western Blot membrane?

Do you know why my membrane looks like this? I did this western for Anti-hnRNP D/AUF1 antibody ab50692, as primary antibody in 1:1000 overnight at 4c and Anti-rabbit IgG HRP linked antibody...

07 February 2021 5,591 4 View

What is the best bioinformatic tool to predict the interaction of peptide with bacterial cell wall membrane?

Hello everyone, How are you guys doing? I'm currently working with antimicrobial peptide (AMP) project and one of our objectives is to predict the interaction of peptide against bacterial cell...

01 February 2021 1,351 3 View

How do i calculate the mean of patient age represented by year + months?

Dear all , I'm doing a pediatric study and the majority of sample's ages are repressented by year + months such as 6Y+4M. How can enter the data and calculate the mean in SPSS? Thank you for...

01 February 2021 570 10 View

What are some good protocols for native outer membrane protein extraction from E. coli?

I am trying to extract any and all outer membrane proteins from a lab strain of e. coli (ATCC 11229) and am looking for the best general protocol to extract outer membrane proteins. All...

25 January 2021 5,736 3 View