I've been trying to look at GABA expression in the lateral hypothalamus but my IHC never works for this brain area. I used rabbit anti-GABA (Sigma Aldrich, A2052) which has been cited nearly 600 times. The interesting thing is that I can detect GABA cells in other brain area using this antibody (e.g in the amygdala) but not in lateral hypothalamus. The image in this area is just so noisy with lots of background.

Thank you.