I am trying to find studies performed on heavy metals, namely Pb, interfering with the use of DCFH-DA for ROS measurement in living cells. I have found some studies about nanomaterials quenching DCF florescence. I also know that there is a modified DCF for the quantification of divalent cations (and Pb). But I haven't found direct studies of Pb effect on DCF fluorescence.
Thank you! I already did, since I did not find straight forward information in the literature. Pb quenches DCF fluorescence at high DCF concentrations (1-10 uM), but augments it at lower DCF concentrations (no effect seen at 0.1 uM DCF). Varying Pb concentration (50 and 100 uM) does not change the picture. Finally I concluded that the effect of quenching in my results is not significant, especially when my ROS experiments with DCFH2-DA are only qualitiative.
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