Has anyone worked with ELISA for Activation Induced Deaminase (AID) in human plasma?

More Caroline Rönnberg's questions See All

Book/article recommendations on international climate finance?

Hi y’all! I’m currently trying to grasp the complex world of climate finance, especially in the context of the promises in the Paris Agreement of increasing flows of finance to low- and middle...

08 March 2024 9,187 2 View

Immunohistochemistry + histochemistry on the same histological section?

Hey everyone, has anyone ever performed immunohistochemical staining followed by histochemical staining on the same histological section (like a double labeling)?

20 August 2023 3,887 8 View

Mouse NMJ staining - size of muscle fibre bundles?

I am hoping someone here can give me a little help with my NMJ staining. I am staining mouse so keys and tibialis anterior muscle. The stain works well but even though I have teased the muscles...

08 June 2023 8,347 1 View

How can I install Amber20 with CUDA on Ubuntu 20.04?

For Ubuntu 20.04 I can only install from CUDA 11.x onwards, But Amber asks for up to CUDA 10.2.

20 May 2023 9,071 1 View

What should I pay attention more in RNA extraction from sweet cherry?

I am new to extracting RNA from fruit flesh. Currently, I'm extracting RNA from sweet cherry flesh using Fruitmate and Nucleospin Takara Bio. I have tried using RNeasy kit Qiagen but I failed (but...

16 March 2023 4,826 2 View

How to dowload Magnetic Resonance Image Based Atlas of the Rabbit Brain for Automatic Parcellation?

Good afternoon, The link provided in the article to download the atlas no longeer works. Do you kown if an other way exists to download it? Thank you for your help!

16 February 2023 5,721 1 View

Can I model marine species distribution using MaxEnt and WORLDCLIM climate data?

My species of interest is a marine animal, and my study area is the Mediterranean Sea. All previous occurrence points are within the Mediterranean Sea, and my climate data has been clipped down to...

25 January 2023 5,726 3 View

How to evidence the presence of cellulose or chitin nanofibers on polymeric substrate ?

I would like to check if I achieve to graft/coat cellulose or chitin nanofibers on different substrate (polymers and textiles). Do you know which method would be the best ? I tried already IR...

21 December 2022 8,754 2 View

Hello all? What should the reaction set up for digesting a plasmid PCR product with Dpn1 to remove methylation sites be?

I have been trying to linearize my backbone vector by PCR followed by digestion with Dpn1 restriction enzyme to remove methylation sites before ligation. After PCR, I did gel electrophoresis and I...

08 December 2022 1,969 6 View

Is it possible to study marine microplastics from Bongo nets deployed obliquely?

I found a lot of microplastics in my zooplankton samples and I'm wondering if that could be useful, or if the only way to quantify MP is manta net.

28 August 2022 8,474 3 View

What precautions should be taken while handling S. aureus enterotoxin Type B in the lab?

I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...

07 August 2024 6,034 3 View

How do soil microflora interact with plant roots and influence plant nutrition, health, and productivity?

06 August 2024 9,618 3 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How does the Retention time effect the Biogas Production?

List down the various factors responsible and how does this effect the overall biogas production when it is related to Hydraulic retention time of the digester based on the feedstock?

27 July 2024 4,800 4 View

What does negative readings in spectrophotometer denote. Was testing TSS content of bacterial cultures?

I was performing estimation of Total Soluble Sugar content in bacterial cultures by Anthrone method. Out of 9 samples, only one sample showed positive reading while others had a negative reading...

22 July 2024 9,576 4 View

What are the strategies to Enhance IgG-Producing Plasma Cells in mice for Monoclonal Antibody Development?

I have immunized BalB/C mice with a protein using the intradermal (ID) method with Complete Freund's Adjuvant (CFA) and Incomplete Freund's Adjuvant (IFA), following a 14-day interval and three...

22 July 2024 9,160 2 View

How do I prepare a working stock solution of 14C sodium bicarbonate to get a final activity of 3uCi?

I need to make a stock solution for radioisotopic incubations of freshwater sediments to study dark primary productivity but I have some confusion as to how I calculate the concentration of 14C...

15 July 2024 5,898 0 View

Isolation of Hydrogen Producing Bacteria?

any good research which can show selective microbial strains for producing hydrogen? Yes the normal procedure is known but the isolation is the key for such selective microbial strains which can...

13 July 2024 2,255 3 View

Hi. Can I get the list of publishers for microbiology-related short communications without publication fee (APC)?

I need to know which journals are exempt from publication fees (APC) in the area of microbiology

11 July 2024 6,346 2 View

What are the regulatory guidelines to submit a microorganism as Generally Regarded as Safe ?

Give in detail with available literature and website links for submissions

27 June 2024 9,753 1 View